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Cyclin H Regulates Lung Cancer Progression as a Carcinoma Inducer
Computational and Mathematical Methods in Medicine ( IF 2.809 ) Pub Date : 2021-03-10 , DOI: 10.1155/2021/6646077
Lili Mao 1 , Xu Ling 1 , Ji Chen 2
Affiliation  

Introduction. Studies have previously shown that Cyclin H (CCNH) is involved in the tumorigenesis and development of many cancers. The increasing research in CCNH is associated with the poor prognosis of most human cancers. Early diagnosis and clinical treatment are still the main challenges for lung cancer treatment. However, the exact role of CCNH in the tumorigenesis of lung cancer remains unclear. Methods. The Tumor Genome Atlas (TCGA) database and the Clinical Proteomics Tumor Analysis Association (CPTAC) database were analyzed to detect key genes that might play an important role in lung cancer. The biological functions of CCNH were further revealed through bioinformatics experiments. The Kaplan-Meier method was applied to explore the relationship between CCNH expression and prognosis. Quantitative reverse transcription-polymerase chain reaction (qRT-PCR) was used to detect the expression levels of CCNH in 6 lung cancer tissues and 3 cancer cell lines. The effect of CCNH expression on lung cancer progression was studied by in vitro functional experiments. Results. Database analysis screened out candidate oncogenes, and CCNH was of great significance to the tumorigenesis of lung cancer. The higher the expression of CCNH was, the lower the survival rate of lung cancer patients were. The qRT-PCR data illustrated that the CCNH expression level was largely increased in lung cancer tissues and cells. The reduction of CCNH inhibited cell proliferation, invasion, and migration. Conclusion. CCNH was related to poor prognosis, suggesting that CCNH regulated the tumorigenesis and development of lung cancer. Our study suggested that CCNH was a promising biomarker and target in the treatment of lung cancer.

中文翻译:

Cyclin H 作为癌诱导剂调节肺癌进展

介绍。先前的研究表明,Cyclin H (CCNH) 与许多癌症的肿瘤发生和发展有关。对 CCNH 越来越多的研究与大多数人类癌症的不良预后有关。早期诊断和临床治疗仍然是肺癌治疗的主要挑战。然而,CCNH在肺癌肿瘤发生中的确切作用仍不清楚。方法. 对肿瘤基因组图谱(TCGA)数据库和临床蛋白质组学肿瘤分析协会(CPTAC)数据库进行了分析,以检测可能在肺癌中起重要作用的关键基因。通过生物信息学实验进一步揭示了CCNH的生物学功能。应用Kaplan-Meier方法探讨CCNH表达与预后的关系。采用定量逆转录聚合酶链反应(qRT-PCR)检测CCNH在6个肺癌组织和3个癌细胞系中的表达水平。通过体外功能实验研究了CCNH表达对肺癌进展的影响。结果. 数据库分析筛选出候选癌基因,CCNH对肺癌的发生具有重要意义。CCNH的表达越高,肺癌患者的生存率越低。qRT-PCR数据表明CCNH表达水平在肺癌组织和细胞中大大增加。CCNH的减少抑制细胞增殖、侵袭和迁移。结论。CCNH与预后不良有关,提示CCNH调控了肺癌的肿瘤发生和发展。我们的研究表明,CCNH 是一种很有前途的肺癌治疗生物标志物和靶点。
更新日期:2021-03-10
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