Lignification is a major cell wall modification that often results in the formation of sophisticated subcellular patterns during plant development or in response to environmental stresses. Precise localization of the spatiotemporal deposition of lignin is of great importance for revealing the lignification regulatory mechanism of individual cells. In loquat fruits, lignification typically increases the flesh lignin content and firmness, reducing their edibility and processing quality. However, the precise localization of the spatiotemporal active zones of lignification inside loquat fruit flesh remains poorly understood, and little is known about the contribution of patterned lignification to cell wall structure dynamics and the subsequent fruit-quality deterioration. Here, we performed an emerging bioorthogonal chemistry imaging technique to trace the in vivo patterned lignification dynamics in cells of loquat fruit flesh during development and storage. In developing fruits, lignified cells (LCs) and vascular bundles (VBs) were the zones of active lignification, and ring-like LCs deposited lignin at both the inner wall layer and the outer periphery sides. The domino effect of the generation of LCs was preliminarily visualized. In mature fruits, the newly formed lignin in the flesh of fruits during storage was specifically deposited in the corners and middle lamellae of parenchyma cells surrounding the VBs, resulting in the development of a reticular structure. Based on the findings, distinct spatiotemporal patterned lignification modes for different flesh cells in loquat fruits were proposed. These findings provide loquat lignification dynamics together with spatiotemporal data that can improve our understanding of the lignification process in planta.

中文翻译：

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用 3-O-炔丙基咖啡醇进行生物合成标记揭示了枇杷果实在发育和采后贮藏过程中的体内细胞特异性图案化木质化

木质化是一种主要的细胞壁修饰，通常导致在植物发育过程中或对环境胁迫作出反应时形成复杂的亚细胞模式。木质素时空沉积的精确定位对于揭示单个细胞的木质化调控机制具有重要意义。在枇杷果实中，木质化通常会增加果肉木质素含量和硬度，降低其可食用性和加工质量。然而，对于枇杷果肉内部木质化的时空活性区域的精确定位仍然知之甚少，对于图案化木质化对细胞壁结构动力学和随后的果实品质恶化的贡献知之甚少。这里，我们进行了一种新兴的生物正交化学成像技术，以追踪在发育和储存过程中枇杷果肉细胞中的体内图案化木质化动力学。在果实发育过程中，木质化细胞（LCs）和维管束（VBs）是木质化活跃的区域，环状LCs在内壁层和外周侧均沉积木质素。LCs产生的多米诺骨牌效应初步显现。在成熟果实中，贮藏过程中果实果肉中新形成的木质素特异地沉积在VBs周围薄壁细胞的角部和中片层中，从而形成网状结构。基于这些发现，提出了枇杷果实中不同肉细胞的不同时空模式化木质化模式。在足底.