Background

Affective disorders (AD) have been associated with a higher prevalence of the gut Flavonifractor genus and a lower abundance of the gut Christensenellaceae family.

Objective and methods

By pooling two independent study samples of patients with AD (n = 176), their unaffected first-degree relatives (n = 70) and healthy controls (n = 101) we aimed to replicate and extend our prior findings of differential Flavonifractor prevalence and Christensenellaceae abundance when comparing patients with AD and healthy controls. The gut microbiota was profiled using 16S rRNA gene amplicon sequencing.

Results

The pattern of higher prevalence of Flavonifractor and lower Centered Log-Ratio (CLR) abundance of Christensenellaceae was associated with AD. In generalized linear models the CLR abundance of Christensenellaceae was lower in patients with AD (p = 0.024), and in smokers (p = 1.9*10⁻⁴), and inversely associated with increasing waist circumference (p = 0.031). The prevalence of Flavonifractor was higher in patients with AD (p = 0.033) and in smokers (p = 0.036). No impact of psychotropic medication was found. The CLR abundance of Christensenellaceae (p = 0.041), but not the prevalence of Flavonifractor (p = 0.20) could distinguish non-smoking patients with AD from non-smoking healthy controls, whereas no such associations were found in smokers. Unaffected relatives neither differed from patients with AD nor from healthy controls.

Conclusion

Compared with findings in healthy controls, AD was associated with a significantly lower CLR abundance of the health-linked Christensenellaceae and a significantly higher prevalence of Flavonifractor; findings that are associated with enhanced oxidative stress and systemic low-grade inflammation. If our observations are validated in future independent studies, they support the notion that parts of aberrant gut microbiota are shared by AD and states of dysmetabolism.

背景

情感障碍 (AD) 与肠道Flavonifractor属的较高患病率和肠道 Christensenellaceae 家族的较低丰度有关。

目的和方法

通过汇集 AD 患者 ( n  = 176)、他们未受影响的一级亲属 ( n  = 70) 和健康对照 ( n  = 101) 的两个独立研究样本，我们旨在复制和扩展我们先前关于差异黄酮类化合物患病率和 Christensenellaceae的研究结果比较 AD 患者和健康对照者时的丰度。使用 16S rRNA 基因扩增子测序分析肠道微生物群。

结果

Flavonifractor的较高流行和Christensenellaceae较低的中心对数比 (CLR) 丰度的模式与 AD 相关。在广义线性模型中，AD 患者 ( p  = 0.024) 和吸烟者 ( p  = 1.9*10 ^-4 ) 的克里斯滕森菌科的 CLR 丰度较低，并且与腰围增加呈负相关 ( p  = 0.031)。AD 患者 ( p  = 0.033) 和吸烟者 ( p  = 0.036)的Flavonifractor患病率较高。没有发现精神药物的影响。Christensenellaceae 的 CLR 丰度 ( p  = 0.041)，但不是Flavonifractor ( p  = 0.20) 可以区分不吸烟的 AD 患者和不吸烟的健康对照组，而在吸烟者中没有发现这种关联。未受影响的亲属与 AD 患者和健康对照均无差异。

结论

与健康对照组的发现相比，AD 与与健康相关的 Christensenellaceae 的 CLR 丰度显着降低和Flavonifractor的流行率显着升高有关；与增强的氧化应激和全身性低度炎症相关的发现。如果我们的观察结果在未来的独立研究中得到验证，它们就会支持 AD 和代谢异常状态共有部分异常肠道微生物群的观点。