The expression of a gene encoding peroxisomal Cu-Zn superoxide dismutase from Saussurea involucrata Kar. et Kir. was induced by low temperature, PEG6000 treatment, and NaCl stress. To investigate the role of SikCuZnSOD3 in the mitigation of abiotic stress, we used Agrobacterium-mediated transformation to create transgenic cotton that overexpressed SikCuZnSOD3. Phenotypic analysis of T₄ generation transgenic lines showed that they generally grew better than wild-type cotton under low temperature, PEG6000 treatment, and NaCl stress. Although there were no significant differences under control conditions, transgenic plants exhibited greater survival, fresh weight, and dry weight than wild-type plants under all three stress treatments. Additional physiological analyses demonstrated that the transgenic cotton had higher relative water content, proline and soluble sugar contents, and activity of antioxidant enzymes (superoxide dismutase, catalase, and peroxidase), as well as lower relative conductivity, malondialdehyde content, and H₂O₂ and O₂⁻ accumulation. More importantly, overexpression of SikCuZnSOD3 increased the yield of cotton fiber. Our results confirm that the overexpression of SikCuZnSOD3 can improve the abiotic stress resistance of cotton by increasing the activity of antioxidant enzymes, maintaining ROS homeostasis, and reducing cell membrane damage.

雪莲中编码过氧化物酶体铜锌超氧化物歧化酶的基因的表达。等基尔。是由低温、PEG6000 处理和 NaCl 胁迫诱导的。为了研究SikCuZnSOD3在缓解非生物胁迫中的作用，我们使用农杆菌介导的转化来创建过度表达SikCuZnSOD3的转基因棉花。T ₄代转基因品系的表型分析表明，在低温、PEG6000 处理和 NaCl 胁迫下，它们普遍比野生型棉花生长得更好。尽管在对照条件下没有显着差异，但转基因植物在所有三种胁迫处理下均表现出比野生型植物更高的存活率、鲜重和干重。进一步的生理分析表明，转基因棉花具有较高的相对含水量、脯氨酸和可溶性糖含量以及抗氧化酶（超氧化物歧化酶、过氧化氢酶和过氧化物酶）活性，以及​​较低的相对电导率、丙二醛含量和H ₂ O ₂和O ₂ ⁻积累。更重要的是，SikCuZnSOD3的过度表达增加了棉纤维的产量。我们的结果证实， SikCuZnSOD3的过表达可以通过增加抗氧化酶的活性、维持ROS稳态、减少细胞膜损伤来提高棉花的非生物胁迫抗性。