Herein we are reporting, the in-situ synthesis of polydopamine capped silver nanoparticles (PDA-AgNPs) and its cysteamine (CYS) and tetracycline (TC) drugs sensor applications. The synthesized PDA-AgNPs was characterized by UV–Visible, Fourier Transformed–Infrared, X-Ray Diffraction, Zeta Potential, and High Resolution-Transmission Electron Microscope techniques. We have utilized PDA-AgNPs as probes for the detection of CYS and TC by spectrophotometry method. The addition of CYS has led to a decrease in the absorption of PDA-AgNPs. The yellow color solution becomes colorless and observed the aggregated particles in the TEM image. In contrast, the addition of TC, into the same solution, absorption of PDA-AgNPs was increased. The color of the solution became dark yellow and the particles are obtained de-aggregation. Based on the Surface Plasmon Resonance (SPR) band absorption changes, the detection limit of CYS and TC was calculated to be 180 nM and 17 nM, respectively. Interestingly, 600-fold of common interferences did not interfere with the detection of CYS and TC. PDA-AgNPs was successfully utilized to detect CYS and TC in serum, urine, and drug samples. Finally, we have prepared a paper-based colorimetric kit for on-site monitoring of CYS and TC.

在本文中，我们报道了聚多巴胺封端的银纳米颗粒（PDA-AgNPs）及其半胱胺（CYS）和四环素（TC）药物传感器应用的原位合成。合成的PDA-AgNPs具有紫外可见，傅立叶变换红外，X射线衍射，Zeta电位和高分辨率透射电子显微镜技术的特征。我们已经利用PDA-AgNPs作为探针通过分光光度法检测CYS和TC。CYS的添加导致PDA-AgNPs的吸收减少。黄色溶液变为无色，并且在TEM图像中观察到聚集的颗粒。相反，在同一溶液中添加TC可以增加PDA-AgNPs的吸收。溶液的颜色变为深黄色，并且使颗粒解聚。根据表面等离子体共振（SPR）谱带吸收变化，CYS和TC的检出限分别计算为180 nM和17 nM。有趣的是，600倍的常见干扰不干扰CYS和TC的检测。PDA-AgNPs已成功用于检测血清，尿液和药物样品中的CYS和TC。最后，我们准备了一个基于纸的比色试剂盒，用于现场监测CYS和TC。