The chromosomal locations of a new class of Revolver transposon-like elements were analyzed by using FISH method on the metaphase chromosome in somatic cell division of the rye cultivar Petkus. First, the Revolver standard element probe λ2 was weakly hybridized throughout the rye chromosome, and comparatively large interstitial signals spotted with a dot shape were detected together with several telomeric regions. The dot shape interstitial signal was stably detected at one site on Chromosome (Chr) 1R (middle part of the interstitial region of the short arm), three sites on Chr 2R (distal part of the interstitial region and adjacent to the centromere on the short arm, middle part of the interstitial region of the long arm), and two sites on Chr 5R (middle part of the interstitial region and adjacent to the centromere on the long arm). The Revolver λ2 probe was effective for identification of 1R, 2R, and 5R chromosomes. On the other hand, Revolver nonautonomous element-specific L626-BARE-100 probe was strongly distributed throughout the rye chromosomes, and considerable numbers and diverse lengths of transcripts were detected by RT-PCR. Although the standard elements were found in localized clusters, the nonautonomous elements tended to be dispersed throughout the genome. Clustered nature of Revolver is a significantly rare case in genomics.

采用FISH方法对黑麦品种Petkus体细胞分裂中期染色体进行了一类新的Revolver转座子样元件的染色体定位分析。一、左轮手枪标准元素探针λ2在整个黑麦染色体中弱杂交，并且检测到较大的点状间隙信号和几个端粒区域。在Chromosome (Chr) 1R上1个位点（短臂间质区中部）、Chr 2R上3个位点（间质区远端和短臂着丝粒附近）稳定检测到点状间隙信号。臂，长臂间质区的中间部分）和 Chr 5R 上的两个位点（间质区的中间部分，与长臂上的着丝粒相邻）。所述左轮λ2探针是有效的1R，2R，5R和染色体的鉴定。另一方面，左轮手枪非自主元件特定 L626-BARE- 100 探针强烈分布在整个黑麦染色体中，并且通过 RT-PCR 检测到相当数量和不同长度的转录物。尽管在局部簇中发现了标准元件，但非自主元件往往分散在整个基因组中。左轮手枪的聚类性质在基因组学中非常罕见。