Abstract

A key component of the recently described bioluminescent system of higher fungi is luciferase, a new class of proteins. The properties of fungal luciferase and their relationship with its structure are interesting both for improving autoluminescent systems already created on its basis and for creating new ones. Therefore, it is extremely important to understand the spatial structure of this protein. We have performed heterologous expression and purification of Neonothopanus nambi luciferase, obtained a protein suitable for subsequent crystallization, and also determined some biochemical properties of the recombinant luciferase.

中文翻译：

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真菌Nonnothopanus nambi的重组荧光素酶的获取和性质

摘要

最近描述的高级真菌生物发光系统的关键组件是萤光素酶，这是一类新的蛋白质。真菌荧光素酶的性质及其与结构的关系，对于改善已经在其基础上创建的自发光系统以及创建新的发光系统均很有趣。因此，了解这种蛋白质的空间结构极为重要。我们已经进行了新Nothohopanus nambi荧光素酶的异源表达和纯化，获得了适合随后结晶的蛋白质，还确定了重组荧光素酶的一些生化特性。