Abstract

The current study evaluated Saussurea lappa extract (SLE) as possible therapeutic agent for osteoarthritis treatment in mice model. Male BALB/c nude mice were separated into sham, model and SLE treatment (at 1.0, 1.5, 2.0, 2.5, and 3.0 mg/kg) groups. Osteoarthritis mice model was prepared by injecting 5 mg/kg doses of monosodium iodoacetate (MIA) to mice via intra-articular route. The SLE was injected to mice for 20 days from day 2 of MIA injection through intraperitoneal route. The SLE treatment alleviated OA-induced higher secretion of interleukin-6, TNF-α, and IL-1β in mice serum. Moreover, elevated levels of P2X7R and MMP-13 in OA mice were also significantly down-regulated on treatment with SLE. In OA mice SLE treatment suppressed expression of SP and PGE2 in cartilage tissues. The expression of activated IκBα and NF-κB p65 was also inhibited markedly by SLE treatment in OA mice. In summary, SLE has protective effect on osteoarthritis in mice by targeting interleukin overproduction and P2X7R expression. Besides, it suppressed MMP expression and deactivated NF-κB signaling pathway. Therefore, SLE can be developed for the treatment of osteoarthritis.

中文翻译：

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雪莲提取物通过抑制NF-κB途径对小鼠骨关节炎的预防作用

摘要

当前的研究评估了雪莲提取物（SLE）作为小鼠模型中骨关节炎治疗的可能治疗剂。将雄性BALB / c裸鼠分为假，模型和SLE处理组（分别为1.0、1.5、2.0、2.5和3.0 mg / kg）。通过经由关节内途径向小鼠注射5mg / kg剂量的碘乙酸单钠（MIA）来制备骨关节炎小鼠模型。从MIA注射的第2天起，通过腹膜内途径将SLE注射给小鼠20天。SLE治疗减轻了OA诱导的小鼠血清中白介素6，TNF-α和IL-1β的更高分泌。此外，OA小鼠中P2X7R和MMP-13的升高水平在用SLE治疗后也显着下调。在OA小鼠中，SLE治疗可抑制软骨组织中SP和PGE2的表达。SLE治疗还可以明显抑制OA小鼠体内活化的IκBα和NF-κBp65的表达。总而言之，SLE通过靶向白细胞介素过量生产和P2X7R表达，对小鼠骨关节炎具有保护作用。此外，它抑制了MMP表达，并使NF-κB信号通路失活。因此，可以开发SLE来治疗骨关节炎。