Potato affecting viruses is an important biotic factor that hampers both the quality and yield of potato production worldwide. The occurrence of potato viruses has been historically recorded in India. Viruses such as potato virus X (PVX), potato virus Y (PVY), potato virus A (PVA), potato virus M (PVM), potato virus S (PVS), potato leafroll virus (PLRV) have been recorded in all potato growing zones. To investigate the presence and distribution of potato virus diseases in North-East India, a survey of seven major potato growing regions was conducted during the 2017 and 2018 growing seasons. The presence of potato viruses was confirmed by double antibody sandwich ELISA (DAS-ELISA) with reverse transcriptase polymerase chain reaction (RT-PCR) performed on selected samples. The sequencing followed by annotation of PCR amplicons obtained from RT-PCR showed 95–100% nucleotide sequence identities with respective virus accessions on NCBI GenBank. The multiple sequence analysis and phylogenetic studies provided an overview of the genetic diversity pattern among different potato virus isolates. This study reveals a broad picture of the potato virus infections occurring in Northeastern states of India, which may be useful to develop effective control strategies against the potato viruses.

中文翻译：

---

印度东北部马铃薯病毒的鉴定、流行和遗传多样性研究

影响马铃薯的病毒是影响全球马铃薯生产质量和产量的重要生物因素。马铃薯病毒的发生在印度历史上已有记载。马铃薯病毒X（PVX）、马铃薯病毒Y（PVY）、马铃薯病毒A（PVA）、马铃薯病毒M（PVM）、马铃薯病毒S（PVS）、马铃薯卷叶病毒（PLRV）等病毒已在所有马铃薯中均有记录。生长区。为了调查印度东北部马铃薯病毒病的存在和分布，在 2017 年和 2018 年的生长季节对七个主要马铃薯种植区进行了调查。马铃薯病毒的存在通过双抗体夹心 ELISA (DAS-ELISA) 和对选定样品进行的逆转录酶聚合酶链反应 (RT-PCR) 确认。测序和从 RT-PCR 获得的 PCR 扩增子的注释显示 95-100% 核苷酸序列同一性与 NCBI GenBank 上的相应病毒登录。多序列分析和系统发育研究概述了不同马铃薯病毒分离株之间的遗传多样性模式。这项研究揭示了印度东北部各州发生的马铃薯病毒感染的广泛情况，这可能有助于制定针对马铃薯病毒的有效控制策略。