In grapevines, the frequent germination of prompt buds always leads to tedious task during summer management in vineyards. Strigolactones have been discovered to play an essential role in regulating shoot branching in different plant species. We investigated the effect of strigolactones (SLs) on grapevine prompt bud outgrowth. Two-year-old grape cuttings (Vitis vinifera L.) with non-burst prompt buds were used as material and subjected to decapitation (control group), or decapitation with subsequent GR24 (Germination releaser 24) treatment (GR), an analog of exogenous SLs. Results showed that grapevine bud growth was significantly induced at nodes 1 and 2, but was largely inhibited by GR24 after decapitation. Transcriptome results revealed the involvement of numerous genes associated with hormone synthesis and signal transduction. GR24 treatment could modulate IAA content and inhibit NCED decrease caused by decapitation, which is the key gene involved in ABA biosynthesis, possibly via BRC1-mediated pathway. Key genes participating in BR biosynthesis were largely modulated. The regulation of different hormones (CTK and BR) on cell cycle genes may be another important mechanism of GR24 regulation on grapevine bud outgrowth. Our results could provide new avenues for research and reveal hormone (IAA, ABA, CTK, and BR) signaling response to SLs in managing shoot branching in grapevines.

在葡萄藤中，迅速发芽的频繁发芽总是导致葡萄园夏季管理过程中繁琐的工作。已经发现，Strigolactones在调节不同植物物种的枝条分支中起着至关重要的作用。我们调查了strigolactones（SLs）对葡萄迅速芽生长的影响。两岁的葡萄插条（Vitis viniferaL.）具有非爆裂的短芽作为材料，并进行断头术（对照组），或随后进行GR24（发芽释放剂24）处理（GR）进行断头术（GR）（外源SL的类似物）。结果显示，断头后，葡萄藤芽的生长在节点1和2处被明显诱导，但在很大程度上被GR24抑制。转录组结果揭示了与激素合成和信号转导相关的众多基因的参与。GR24处理可调节IAA含量并抑制由断头引起的NCED降低，断头可能是通过BRC1参与ABA生物合成的关键基因。介导的途径。参与BR生物合成的关键基因受到很大的调节。细胞周期基因上不同激素（CTK和BR）的调控可能是GR24调控葡萄芽生长的另一重要机制。我们的结果可能为研究提供新途径，并揭示激素（IAA，ABA，CTK和BR）对SLs的信号响应，从而控制葡萄树的枝条分支。