An effective culture system for producing embryos was developed from isolated microspores of sweet pepper (Capsicum annuum L.). The present work aimed to define exogenous arabinogalactan proteins (AGPs) on the embryogenesis of sweet pepper under the growth environments of donor plants. The experiments were performed with the cultivars Arancia, California wonder, Californium, Camelot, Inspiration, Plato, and Taranto under three different growth environments of the donor plants. The impacts of the different numbers of sweet pepper ovaries (0, 5, 10, and 15) were evaluated on microspore embryogenesis of cultivars in a factorial design with five replications. The simultaneous application of 10 ovaries in stress (sucrose starvation; temperature) and induction media resulted in the highest embryogenesis of the seven aforementioned cultivars under field conditions. Also, the impacts of 0, 80, 120, and 200 mg L⁻¹ gum arabic were studied on embryogenesis in stress and induction media. The treatments containing 120 mg L⁻¹ gum arabic showed the highest embryogenesis across all cultivars, except the California Wonder cultivar in the field conditions. Also, the simultaneous impact of the number of ovaries and concentrations of gum arabic on embryogenesis were studied. A combination of ten ovaries and 120 mg L⁻¹ gum arabic generated the highest embryogenesis in the majority of cultivars. The results revealed that ovaries of sweet pepper and gum arabic had a significant impact on the efficiency of microspore embryogenesis in sweet pepper plants under field conditions.

从甜椒（Capsicum annuum）的分离小孢子中建立了一种有效的产生胚胎的培养系统。L.）。本工作旨在定义供体植物生长环境下甜椒胚发生的外源阿拉伯半乳聚糖蛋白（AGP）。实验是在供体植物的三种不同生长环境下，对加利福尼亚州的Arancia，Californium，Camelot，Inspiration，Plato和Taranto品种进行的。在有五个重复的因子设计中，评估了不同数量的甜椒卵巢（0、5、10和15）对小孢子胚胎发生的影响。在胁迫（蔗糖饥饿；温度）和诱导培养基中同时施用10个卵巢导致在田间条件下上述七个品种的最高胚发生。另外，0、80、120和200 mg L ^-1的影响研究了阿拉伯树胶在应激和诱导培养基中的胚胎发生。含有120 mg L ^-1阿拉伯树胶的处理在所有栽培品种中均表现出最高的胚发生率，但在田间条件下，除了California Wonder品种以外。此外，还研究了卵巢数量和阿拉伯胶浓度对胚胎发生的同时影响。在大多数品种中，十个卵巢和120 mg L ^-1阿拉伯树胶的组合产生最高的胚发生。结果表明，田间条件下，甜椒和阿拉伯树胶的卵巢对甜椒植株中的小孢子胚发生效率有显着影响。