Marek’s disease (MD), a highly contagious T cell lymphoid neoplasia disease of chickens, causes huge economic losses to the poultry industry. It is the only one tumor disease which can be prevented by vaccine in chickens; therefore, MD is considered to be an excellent model to study the pathogenesis of virus-induced cancer. Recently, abundant evidences have verified that miRNAs are regulators in the process of neoplastic transformation. In our previous study on miRNome analysis of MDV-induced lymphoma in chicken, we found that gga-miR-181a was downregulated drastically in MDV-infected spleens. To further investigate the role of gga-miR-181a in MDV-induced lymphomagenesis, we performed cell migration assay, and the results suggested that gga-miR-181a suppressed the migration of MDV-transformed lymphoid cell (MSB-1). Subsequently, luciferase reporter gene assay revealed that acidic nuclear phosphoprotein 32A (ANP32A) was a functional target gene of gga-miR181a. Real-time PCR and western blot assay showed that the mRNA and protein levels of ANP32A were downregulated in gga-miR-181a mimic group at 48-h and 96-h post-transfection, respectively, indicating that ANP32A was modulated by gga-miR-181a. All the results suggested that gga-miR-181a was an inhibitor in MSB-1 cell migration. ANP32A was a direct target gene of gga-miR-181a and they were implicated in MD lymphoma tumorigenesis.

马立克氏病 (MD) 是一种高度传染性的鸡 T 细胞淋巴肿瘤疾病，给家禽业造成了巨大的经济损失。它是唯一一种可以通过疫苗在鸡中预防的肿瘤疾病；因此，MD被认为是研究病毒诱发癌症发病机制的极好模型。最近，大量证据证实miRNAs是肿瘤转化过程中的调节因子。在我们之前对鸡中 MDV 诱导的淋巴瘤的 miRNome 分析的研究中，我们发现 gga-miR-181a 在 MDV 感染的脾脏中显着下调。为了进一步研究 gga-miR-181a 在 MDV 诱导的淋巴瘤形成中的作用，我们进行了细胞迁移测定，结果表明 gga-miR-181a 抑制了 MDV 转化的淋巴样细胞 (MSB-1) 的迁移。随后，ANP32A ) 是 gga-miR181a 的功能靶基因。实时PCR和蛋白质印迹分析显示，gga-miR-181a模拟组在转染后48-h和96-h时ANP32A的mRNA和蛋白水平分别下调，表明ANP32A受gga-miR调节-181a。所有结果表明gga-miR-181a是MSB-1细胞迁移的抑制剂。ANP32A 是 gga-miR-181a 的直接靶基因，它们与 MD 淋巴瘤的发生有关。