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Selection strategy of a Coffea canephora rootstock with simultaneous nematode resistance to Meloidogyne exigua , M. incognita and M. paranaensis
European Journal of Plant Pathology ( IF 1.8 ) Pub Date : 2021-03-09 , DOI: 10.1007/s10658-021-02225-8
Wallace Gonçalves , Vinicius Teixeira Andrade , Bárbhara Joana dos Reis Fatobene , Larissa de Brito Caixeta , Lilian Padilha , Claudio Marcelo Gonçalves Oliveira , Juliana Magrinelli Osório Rosa , Lucas Mateus Rivero Rodrigues , Oliveiro Guerreiro Filho

The cultivars of most Brazilian Arabica coffee plantations are susceptible to root knot nematodes (RKN), Meloidogyne spp. By planting resistant rootstocks of the species Coffea canephora, a successful strategy in some producing regions, coffee can be cultivated in RKN-infested areas. However, the only currently available rootstock cultivar in Brazil has a segregation rate close to 20% for susceptibility. To breed new rootstock cultivars resistant to the main RKN species, with a lower segregation rate, 10 C. canephora clones were selected in areas infested by these nematodes, with confirmed resistance to Meloidogyne exigua, M. incognita and M. paranaensis, under controlled conditions. The genetic compatibility analysis of the clones detected only one incompatible combination (CcK1xCcR5 and its reciprocal). Almost all F1 hybrids resulting from the cross between the selected clones were resistant to M. exigua and M. incognita. According to the reproduction factor, 77% of the evaluated F1 hybrids proved resistant to M. paranaensis, and those classified as susceptible had variable segregation rates within the progeny, generally lower than 25%. Based on these data, different hybrid combinations can be suggested for the development of new rootstock cultivars of C. canephora with simultaneous resistance to coffee RKN. This approach has immediate practical applications in grafting Arabica coffee scion cultivars in areas where nematodes restrict cultivation.



中文翻译:

同时对线虫对南方根结线虫,南方根结线虫和巴拉那根根结线虫具有抗线虫性的小叶咖啡树砧木的选择策略

大多数巴西的阿拉比卡咖啡种植的品种很容易受到根结线虫(RKN),根结线虫属。通过在某些产区种植耐咖啡树种的抗性砧木,可以在RKN感染地区种植咖啡。但是,巴西目前仅有的砧木品种的易感性分离率接近20%。为了培育对主要RKN种具有抗性的新砧木品种,以较低的隔离率,在这些线虫感染的区域中选择了10个C. canephora克隆,并确认了对Meloidogyne exiguaM。incognitaM. paranaensis的抗性,在受控条件下。克隆的遗传相容性分析仅检测到一种不相容的组合(CcK1xCcR5及其对等)。几乎所有的F 1杂种都是由所选克隆间的杂交产生的,对M. exiguaM. incognita有抗性。根据繁殖因子,评估的F 1杂种中有77%被证明对Paranaensis耐药,而被归类为易感的F 1杂种在子代中具有可变的分离率,通常低于25%。基于这些数据,可以提出不同的杂交组合以开发新的C. canephora砧木品种同时耐咖啡RKN。这种方法在将线虫限制种植的地区嫁接阿拉比卡咖啡接穗栽培品种具有直接的实际应用价值。

更新日期:2021-03-09
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