Cannabidiol (CBD) has proven clinical benefits in the treatment of seizures, inflammation, and pain. The recent legalization of CBD in many countries has caused increased interest in the drug as an over-the-counter treatment for athletes looking to improve recovery. However, no data on the effects of CBD on the adaptive response to exercise in muscle are available. To address this gap, we eccentrically loaded the tibialis anterior muscle of 14 rats, injected them with a vehicle (n = 7) or 100 mg/kg CBD (n = 7), and measured markers of injury, inflammation, anabolic signaling, and autophagy 18 hr later. Pro-inflammatory signaling through nuclear factor kappa B (NF-kB) (Ser536) increased with loading in both groups; however, the effect was significantly greater (36%) in the vehicle group (p < .05). Simultaneously, anabolic signaling through ribosomal protein S6 kinase beta-1 (S6K1) (Thr389) increased after eccentric contractions in both groups with no difference between vehicle and CBD (p = .66). The ribosomal protein S6 phosphorylation (240/244) increased with stimulation (p < .001) and tended to be higher in the CBD group (p = .09). The ubiquitin-binding protein p62 levels were not modulated by stimulation (p = .6), but they were 46% greater in the CBD compared with the vehicle group (p = .01). Although liver weight did not differ between the groups (p = .99) and levels of proteins associated with stress were similar, we did observe serious side effects in one animal. In conclusion, an acute dose of CBD decreased pro-inflammatory signaling in the tibialis anterior without blunting the anabolic response to exercise in rats. Future research should determine whether these effects translate to improved recovery without altering adaptation in humans.

大麻二酚 (CBD) 在治疗癫痫发作、炎症和疼痛方面已被证明具有临床益处。最近许多国家将 CBD 合法化，这引起了人们对该药物的兴趣增加，该药物作为一种非处方药治疗希望改善康复的运动员。然而，没有关于 CBD 对肌肉运动的适应性反应影响的数据。为了解决这个差距，我们偏心加载 14 只大鼠的胫骨前肌，给它们注射载体 ( n  = 7) 或 100 mg/kg CBD ( n = 7)，并在 18 小时后测量损伤、炎症、合成代谢信号和自噬的标志物。通过核因子 kappa B (NF-kB) (Ser536) 的促炎信号传导随着两组的负荷增加；然而，载体组的效果显着更大 (36%) ( p  < .05)。同时，通过核糖体蛋白 S6 激酶 beta-1 (S6K1) (Thr389) 的合成代谢信号在两组离心收缩后增加，载体和 CBD 之间没有差异 ( p  = .66)。核糖体蛋白 S6 磷酸化 (240/244) 随刺激增加 ( p  < .001)，并且在 CBD 组中趋于更高 ( p  = .09)。泛素结合蛋白 p62 水平不受刺激的调节（p = .6)，但与载体组相比，CBD 的患者高出 46% ( p  = .01)。尽管各组之间的肝脏重量没有差异 ( p  = .99)，并且与压力相关的蛋白质水平相似，但我们确实在一只动物身上观察到了严重的副作用。总之，急性剂量的 CBD 降低了胫骨前肌的促炎信号，而不会减弱大鼠对运动的合成代谢反应。未来的研究应该确定这些影响是否会在不改变人类适应能力的情况下转化为改善恢复。