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In vitro human intestinal microbiota biotransformation of nobiletin using liquid chromatography–mass spectrometry analysis and background subtraction strategy
Journal of Separation Science ( IF 3.1 ) Pub Date : 2021-03-07 , DOI: 10.1002/jssc.202001150
Hong-Ci Lan 1 , Shang-Zhen Li 1 , Kai Li 2 , E-Hu Liu 1
Affiliation  

In this study, the in vitro biotransformation of nobiletin by human intestinal microbiota, which is a bioactive polymethoxyflavone widely presented in Citrus plants, has been investigated via utilizing an anaerobic incubation protocol. The incubation samples were detected using high-performance liquid chromatography/quadrupole time-of-flight mass spectrometry. A background subtraction strategy incorporated in Microsoft Office was employed to eliminate the interferences in medium and feces. The parent and three metabolites sinensetin, 5-hydroxy-6,7,3′,4′-tetramethoxyflavone, and 5-demethylnobiletin were detected and identified based on the characteristics of their protonated molecules. The proposed metabolic pathway revealed that nobiletin went through phase I metabolism including demethylation and demethoxylation in human intestinal microbiota. The characterization of nobiletin metabolic profile transformed by human intestinal bacteria would be helpful for understanding its efficacy and action mechanism.

中文翻译:

使用液相色谱-质谱分析和背景扣除策略在体外人肠道微生物群生物转化川川陈皮素

在这项研究中,通过利用厌氧培养方案研究了人肠道微生物群对川陈皮素的体外生物转化,这是一种广泛存在于柑橘植物中的生物活性聚甲氧基黄酮。使用高效液相色谱/四极杆飞行时间质谱法检测孵育样品。Microsoft Office 中包含的背景减除策略用于消除培养基和粪便中的干扰。根据质子化分子的特征,检测并鉴定母体及其三种代谢产物正念珠菌素、5-羟基-6,7,3',4'-四甲氧基黄酮和5-去甲基川胆碱。提议的代谢途径表明川陈皮素在人肠道微生物群中经历了包括去甲基化和去甲氧基化在内的 I 期代谢。
更新日期:2021-03-07
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