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BrRH37, a Cabbage ( Brassica rapa ) DEAD-Box RNA Helicase, Confers Drought Tolerance and ABA Response in Transgenic Arabidopsis Plants
Journal of Plant Biology ( IF 2.9 ) Pub Date : 2021-03-08 , DOI: 10.1007/s12374-021-09306-5
Ghazala Nawaz , Than Zaw Tun Sai , Kwanuk Lee , Su Jung Park , Sy Nguyen Dinh , Hunseung Kang

RNA helicases (RHs) are cellular proteins that help in the regulation of post-transcriptional mRNA metabolism, which is vital for the response of crops to abiotic stresses. In this study, we determined the functional role of a cabbage (Brassica rapa) DEAD-box RH, BrRH37, in abiotic stress and abscisic acid (ABA) responses using transgenic Arabidopsis plants. Although BrRH37 contains a putative chloroplast transit peptide and a potential nuclear localization signal, confocal analysis of the expression of the BrRH37-GFP protein in tobacco leaves revealed that BrRH37 is localized in the nucleus. The transcript levels of BrRH37 in cabbage were increased upon drought, cold, or ABA application, whereas its level was decreased by heat or UV stress. The transgenic Arabidopsis plants that constitutively express BrRH37 showed enhanced seedling and root growth or survival rate under drought stress or upon ABA application, whereas their growth and survival were decreased under cold or UV stress. Notably, transcript levels of ABA biosynthesis-related genes, including ABA1 and ABA3, were significantly increased in the transgenic plants compared with that in the wild type. Importantly, BrRH37 was found to recover the growth of the RNA chaperone-deficient Escherichia coli mutant at low temperature and had the ability to break up base pairs in RNA, suggesting that BrRH37 possesses RNA chaperone activity. Altogether, our results indicate that BrRH37 plays diverse functions in plant response to abiotic stress and ABA possibly via its RNA chaperone activity.



中文翻译:

BrRH37,一种甘蓝(芸苔)DEAD-Box RNA解旋酶,赋予转基因拟南芥植物抗旱性和ABA响应

RNA解旋酶(RHs)是有助于调节转录后mRNA代谢的细胞蛋白,这对于农作物对非生物胁迫的反应至关重要。在这项研究中,我们确定了使用转基因拟南芥植物的甘蓝(芸苔)DEAD-box RH,BrRH37在非生物胁迫和脱落酸(ABA)响应中的功能性作用。尽管BrRH37包含一个假定的叶绿体转运肽和潜在的核定位信号,但共聚焦分析BrRH37-GFP蛋白在烟叶中的表达表明BrRH37位于核中。干旱,寒冷或ABA施用后,白菜中BrRH37的转录水平升高,而热或紫外线胁迫则使其水平降低。转基因组成型表达BrRH37的拟南芥植物在干旱胁迫下或在ABA施用下显示出增强的幼苗和根系生长或存活率,而在寒冷或紫外线胁迫下其生长和存活率降低。值得注意的是,与野生型相比,转基因植物中与ABA生物合成相关基因(包括ABA1ABA3)的转录水平显着提高。重要的是,发现BrRH37可恢复RNA伴侣缺失的大肠杆菌的生长。突变体在低温下具有分解RNA中碱基对的能力,这表明BrRH37具有RNA伴侣活性。总之,我们的结果表明,BrRH37可能通过其RNA伴侣活性在植物对非生物胁迫和ABA的反应中发挥多种功能。

更新日期:2021-03-08
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