Metal combinations have been attracting the attention of scientists for some time. They usually exhibit new characteristics that are different from the ones possessed by their components. In this work, Au/ZnO/Ag nanoparticles were synthesized biologically using Glechoma hederacea L. extract. The synthesized Au/ZnO/Ag nanoparticles were characterized by UV-Vis, Scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR), Transmission electron microscopy (TEM), and Atomic Force Microscopy (AFM). The microscopic methods confirmed the presence of spherical nanoparticles of 50–70 nm. The influence of biologically synthesized Au/ZnO/Ag nanoparticles on the vitality of human cells was evaluated in vitro with the use of established human Acute T Cell Leukemia cell line, Jurkat (ATCC® TIB-152™), as well as mononuclear cells isolated from peripheral blood (PBMC) of voluntary donors. Cell survival and the half-maximal inhibitory concentration index (IC50) were analyzed by the MTT test. The studies showed that the total loss of cell viability occurred at the Au/ZnO/Ag nanoparticle concentration range of 10 µmol–50 µmol. The use of Au/ZnO/Ag nanoparticles at the concentration of 100 µmol eliminated almost all living cells from the culture in 24h. The above observation confirms the result obtained during the MTT test.

一段时间以来，金属组合一直吸引着科学家的注意。它们通常表现出与其组件所具有的特性不同的新特性。在这项工作中，Au/ZnO/Ag 纳米颗粒是使用Glechoma hederacea L.提取物生物合成的。合成的 Au/ZnO/Ag 纳米粒子通过 UV-Vis、扫描电子显微镜 (SEM)、傅里叶变换红外光谱 (FTIR)、透射电子显微镜 (TEM) 和原子力显微镜 (AFM) 进行表征。显微方法证实了 50-70 nm 的球形纳米粒子的存在。体外评价生物合成的Au/ZnO/Ag纳米颗粒对人体细胞活力的影响使用已建立的人类急性 T 细胞白血病细胞系 Jurkat (ATCC® TIB-152™) 以及从自愿捐赠者的外周血 (PBMC) 中分离的单核细胞。通过MTT测试分析细胞存活率和半数最大抑制浓度指数(IC50)。研究表明，在 Au/ZnO/Ag 纳米颗粒浓度范围为 10 µmol–50 µmol 时，细胞活力会完全丧失。使用浓度为 100 µmol 的 Au/ZnO/Ag 纳米颗粒可在 24 小时内消除培养物中的几乎所有活细胞。上述观察证实了在 MTT 测试期间获得的结果。