Lysozyme is a ubiquitous protein and enzyme that prevents the bacterial infections and maintains the catalytic balance in majority of biological fluids. However, it often causes problems at higher concentrations. In particular, the build up of lysozyme engaged with other protein interactions initiates severe disorders in most mammalian cells, such as the formation of harmful aggregates in the nervous system and the loss of connectivity in rheumatism. To understand such complex behaviors with respective to the catalytic activity of the enzyme, the lysozyme solution and the effect of hydrophobic antagonistic salt (NaBPh4) are explored in-vitro, in their relaxation behaviors. Here, we used, both normal dynamic light scattering and home-built in situ AC external electric field light scattering. As results, the fast and slow-mode (with stretched) relaxations are observed in the conventional dynamic light scattering (without an electric field). The results are particularly noteworthy under a low electric field, with robust long-time oscillations, in the scattered intensity correlation function. In addition, the effective interactions are explored by varying the ionic strength of the antagonistic salt: The oscillations are less pronounced, but still clearly represent the ‘underdamped’ motions. Overall, reductions of the relaxations are shown with the applied electric field, with a maximum relaxation occuring at 1mM. In contrast, monotonic decreases of the relaxation rates are shown above 10 mM. Thus, the lysozyme exhibits a charged carrier, responding to ultimate low-frequency oscillations in the scattered correlations. The fit function of long-time oscillation in correlation is presented by an alternating cosine function with a phase, which is related to its possible dynamic elelctrophoretic mobility.

溶菌酶是一种普遍存在的蛋白质和酶，可防止细菌感染并维持大多数生物体液的催化平衡。然而，它通常会在较高浓度下引起问题。特别是，与其他蛋白质相互作用的溶菌酶的积累引发了大多数哺乳动物细胞的严重疾病，例如神经系统中有害聚集体的形成和风湿病的连通性丧失。为了理解这种与酶催化活性相关的复杂行为，溶菌酶溶液和疏水性拮抗盐 (NaBPh4) 的作用在体外进行了松弛行为的探索。在这里，我们使用了正常的动态光散射和自制的原位交流外电场光散射。结果，在传统的动态光散射（没有电场）中观察到快速和慢速模式（具有拉伸）弛豫。结果在低电场下特别值得注意，在散射强度相关函数中具有强大的长时间振荡。此外，通过改变拮抗盐的离子强度来探索有效的相互作用：振荡不太明显，但仍然清楚地代表了“欠阻尼”运动。总体而言，随着施加的电场显示弛豫的减少，最大弛豫发生在 1mM 处。相比之下，松弛率的单调下降显示在 10 mM 以上。因此，溶菌酶表现出带电载体，响应散射相关中的最终低频振荡。相关长期振荡的拟合函数由具有相位的交替余弦函数表示，这与其可能的动态电泳迁移率有关。