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Pericardial fluid and vascular tissue engineering: A preliminary study
Bio-Medical Materials and Engineering ( IF 1 ) Pub Date : 2021-03-02 , DOI: 10.3233/bme-196014
Dilek Sönmezer 1 , Fatma Lati Foğlu 2 , Güler Toprak 2 , Ayhan Düzler 2 , İsmail Alper İşoğlu 3
Affiliation  

BACKGROUND:The heart is surrounded by a membrane called pericardium or pericardial cavity. OBJECTIVE:In this study, we investigated the pericardial fluid (PF) for coating polycaprolactone (PCL) scaffolds. PFS, which is a PF component, was used for coating material. In addition to using PFS for surface coating, MED and fetal bovine serum (FBS) were also used for comparison. METHODS:Pericardial fluid cells (PFSc) isolated from PF were cultured on coated PCL scaffolds for 1, 3 and 5 days. Cell viability was determined using 3-(4, 5-di-methylthiazol- 2-yl)-2, 5-diphenyltetrazolium bromide (MTT) assay. RESULTS:The MTT assay results showed that the viability of cells on PCL scaffold coated with PFS increased over time (P < 0.005), and cell viability was significantly different between PCL scaffolds coated with PFS and non-coated PCL scaffolds. However, cell viability was significantly higher in the PCL scaffolds coated with PFS than non-coated and coated with FBS, MED and PCL scaffolds. Scanning electron microscopy (SEM) microscopy images and MTT assay indicated that PFSc are attached, proliferated, and spread on PCL scaffolds, especially on PCL scaffolds coated with PFS. CONCLUSIONS:These results suggest that PFS is a biocompatible material for surface modification of PCL scaffolds, which can be used as a suitable material for tissue engineering applications.

中文翻译:

心包液和血管组织工程:初步研究

背景:心脏被称为心包或心包腔的膜包围。目的:在本研究中,我们研究了用于涂覆聚己内酯 (PCL) 支架的心包液 (PF)。作为PF成分的PFS用于涂层材料。除了使用 PFS 进行表面涂层外,还使用 ​​MED 和胎牛血清 (FBS) 进行比较。方法:从 PF 中分离的心包液细胞 (PFSc) 在有涂层的 PCL 支架上培养 1、3 和 5 天。使用 3-(4, 5-二甲基噻唑-2-基)-2, 5-二苯基溴化四唑 (MTT) 测定法测定细胞活力。结果:MTT检测结果显示,PFS包被的PCL支架上的细胞活力随时间增加(P<0.005),且PFS包被的PCL支架与未包被的PCL支架的细胞活力有显着差异。然而,与未涂覆和涂覆有 FBS、MED 和 PCL 支架的 PCL 支架相比,涂有 PFS 的 PCL 支架中的细胞活力明显更高。扫描电子显微镜 (SEM) 显微镜图像和 MTT 测定表明 PFSc 在 PCL 支架上附着、增殖和扩散,尤其是在涂有 PFS 的 PCL 支架上。结论:这些结果表明 PFS 是一种用于 PCL 支架表面改性的生物相容性材料,可用作组织工程应用的合适材料。
更新日期:2021-03-07
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