Bone regenerative medicine (BRM) aims to overcome the limitations of conventional treatments for critical bone defects by developing therapeutic strategies, based on temporary bioactive substitutes, capable of stimulating, sustaining, and guiding tissue regeneration. The aim of this study was to validate the “proof of concept” of a cellularized bioactive scaffold and establish its potential for use in BRM. For this purpose, three-dimensional scaffolds of poly-(lactic acid) (PLA), produced by the additive manufacturing technique, were incorporated into a human platelet-rich plasma (PRP-h) fibrin matrix containing human infrapatellar fat pad mesenchymal stem cells (hIFPMSC). The scaffolds (PLA/finbrin-bioactive) were kept under ideal culture conditions in a medium free from fetal bovine serum and analyzed at 5 and 10 days by Scanning Electron Microscopy (SEM), Fourrier Transform Infrared (FTIR), Circular Dichroism and fluorescence microscopy. The results demonstrated the feasibility of obtaining a rigid, cytocompatible, and cellularized three-dimensional structure. In addition, PRP platelets and leukocytes were able to provide a bioactive environment capable of maintaining the viability of hIFPMSC into scaffolds. The results validate the concept of a customizable, bioactive, cellularized, and non-immunogenic strategy for application in BRM.

骨再生医学（BRM）旨在通过开发基于临时生物活性替代物，能够刺激，维持和引导组织再生的治疗策略，克服传统治疗关键性骨缺损的局限性。这项研究的目的是验证细胞化生物活性支架的“概念验证”，并确定其在BRM中的应用潜力。为此，将通过增材制造技术生产的三维聚乳酸（PLA）支架掺入包含人下pat脂肪垫间充质干细胞的人富血小板血浆（PRP-h）纤维蛋白基质中（hIFPMSC）。将支架（PLA / finbrin-bioactive）保持在理想的培养条件下，不含胎牛血清的培养基中，并在5天和10天通过扫描电子显微镜（SEM），傅里叶变换红外（FTIR），圆二色谱和荧光显微镜进行分析。结果证明了获得刚性，细胞相容性和细胞化的三维结构的可行性。另外，PRP血小板和白细胞能够提供能够维持hIFPMSC进入支架的生存能力的生物活性环境。结果验证了可定制，生物活性，细胞化和非免疫原性策略在BRM中的应用概念。和蜂窝化的三维结构。另外，PRP血小板和白细胞能够提供能够维持hIFPMSC进入支架的生存能力的生物活性环境。结果验证了可定制，生物活性，细胞化和非免疫原性策略在BRM中的应用概念。和蜂窝化的三维结构。另外，PRP血小板和白细胞能够提供能够维持hIFPMSC进入支架的生存能力的生物活性环境。结果验证了可定制，生物活性，细胞化和非免疫原性策略在BRM中的应用概念。