Implementation of guidelines to reduce the amount of biodegradable municipal waste (BMW) sent to landfill has created a need in the waste-management industry to investigate possible methods of accelerating biostabilisation of residual BMW. The effect of commercially feasible manipulations (lime and green waste (GW)) on the rate of biostabilisation of the fine (<20 mm) fraction of residual BMW was investigated. The physical and chemical attributes of the composted wastes were measured, and their bacterial communities profiled using traditional culture-based methods. In addition, ammonia-oxidising microbes were monitored during the biostabilisation process using molecular profiling methods. Addition of GW accelerated biostabilisation, reduced conductivity and increased the levels of ammonia-oxidising bacterial (AOB) and archaeal (AOA) genes. The best stability was noted in the dual (Lime + GW) treatment, which was under the limit of 13 mmol O₂ kg DM⁻¹ h⁻¹ recommended by the Irish compost standard. Biostabilised wastes met recommendations for source-segregated compost for pH (6-8) and pathogens (E. coli and Salmonella), but not heavy metals, indicating their unsuitability for uses other than landfill cover. Levels of AOA genes (log 3–6 g⁻¹ DM) were higher than AOB (log 1–6 g⁻¹ DM, indicating AOA may contribute more to potential ammonia oxidation in residual BMW composting.

实施指南以减少送往垃圾填埋场的可生物降解城市垃圾 (BMW) 的数量，这使得垃圾管理行业需要研究加速残留 BMW 生物稳定的可能方法。研究了商业上可行的操作（石灰和绿色废物 (GW)）对残留 BMW 的精细（<20 毫米）部分的生物稳定率的影响。测量了堆肥废物的物理和化学属性，并使用传统的基于培养的方法对其细菌群落进行了分析。此外，在生物稳定过程中使用分子分析方法监测氨氧化微生物。添加 GW 加速了生物稳定性，降低了电导率，并增加了氨氧化细菌 (AOB) 和古细菌 (AOA) 基因的水平。爱尔兰堆肥标准推荐的₂ kg DM ^-1 h ^-1。生物稳定的废物符合针对 pH (6-8) 和病原体（大肠杆菌和沙门氏菌）但不符合重金属的来源隔离堆肥的建议，表明它们不适合用于垃圾填埋场覆盖以外的用途。AOA 基因的水平（log 3-6 g ^-1 DM）高于 AOB（log 1-6 g ^-1 DM，表明 AOA 可能对残留 BMW 堆肥中潜在的氨氧化贡献更大。