Abstract

Coronary artery disease is a multifactorial genetic disease caused by the interaction between genetic and environmental factors. Angiography is the gold standard method for the diagnosis and determining the stage of cardiac disorder. The rs1800588 at the Hepatic Lipase gene and rs1799983 at the endothelial nitric oxide synthase (eNOS) gene are two candidate SNP that result in increased risk of this disease. The aim of this study was to find out the associations of the two mentioned polymorphisms with angiographically proven coronary artery patients in a southern Iranian population. In this study, this two polymorphisms in 287 patients and 229 matched controls were confirmed by angiography and analyzed. Genotype analysis was carried out by PCR and RFLP. Data showed that a significant difference for the eNOS gene polymorphism (p = 0.004) and a non-significant difference for the Hepatic lipase polymorphism (p = 0.261) and increasing severity of angiographic evidences of coronary artery disease were observed. Conclusively the significant association of the G894T with the narrowing of two or three coronary vessels of this patients in an Iranian population have been detected.

摘要

冠状动脉疾病是由遗传和环境因素相互作用引起的多因素遗传病。血管造影是诊断和确定心脏疾病分期的金标准方法。在该rs1800588肝 脂酶基因和rs1799983的内皮型一氧化氮合酶(eNOS) 基因是导致这种疾病风险增加的两个候选 SNP。本研究的目的是找出上述两个多态性与伊朗南部人群中经血管造影证实的冠状动脉患者之间的关联。在这项研究中，这两个多态性在 287 名患者和 229 名匹配的对照中被血管造影证实并分析。通过PCR和RFLP进行基因型分析。数据显示， 观察到eNO S 基因多态性的显着差异( p  = 0.004) 和肝脂肪酶多态性的非显着差异( p = 0.261) 以及冠状动脉疾病血管造影证据的严重程度增加。最终的重要关联在伊朗人群中检测到G894T，该患者的两个或三个冠状血管变窄。