The larval period is a critical stage for many aquatic organisms, because larvae are small, fragile, and have high mortality rates. Amphidromous fish spend their larval stages in the sea, and larval durations vary, possibly reflecting their dispersal tendencies. The genus Rhinogobius (suborder Gobioidei) is widely distributed throughout Asia and exhibits a variety of migratory patterns. Despite some studies of their migratory histories, larval durations of Rhinogobius species are uncertain. This study used analyses of otolith microstructure and trace-elemental chemistry to investigate habitat use throughout the life history of R. nagoyae. Twenty-six adult gobies were collected from seven rivers on Sado Island, in northern Japan, and otolith Sr : Ca ratios were quantified using electron microprobe analysis to infer amphidromous movement. All individuals showed shifts in Sr : Ca ratio across the otolith transect, which indicated movement between freshwater and saltwater. Marine larval duration was estimated by otolith increment counting, combined with Sr : Ca ratio. Larval duration ranged from 29 to 45 days (mean, 35.3), and there were no significant differences among rivers on the island. The relatively short larval duration of R. nagoyae, compared with other amphidromous fish such as ayu and galaxids, may reflect environmental factors such as island size, cool weather or species characteristics.

幼虫期是许多水生生物的关键时期，因为幼虫体形小，易碎，死亡率高。两栖鱼类的幼体在海中度过，幼体的持续时间各不相同，这可能反映了它们的扩散趋势。Rhinogobius属（Gobioidei子亚种）广泛分布于整个亚洲，并表现出各种迁徙模式。尽管对其迁徙历史进行了一些研究，但不确定犀牛物种的幼体持续时间。这项研究使用耳石的微结构和痕量元素化学分析来研究名古屋R的整个生命历史中的栖息地使用情况。从日本北部佐渡岛的7条河流中收集了26只成年虎虾，并使用电子探针分析法定量了耳石Sr：Ca比率，以推断两栖动物的运动。所有个体在耳石横断面都显示出Sr：Ca比值的变化，这表明淡水和盐水之间有运动。通过耳石增量计数结合Sr：Ca比值来估算海洋幼体的持续时间。幼虫的持续时间从29天到45天不等（平均35.3天），岛上河流之间没有显着差异。的相对短的持续时间幼虫R. nagoyae，与其他amphidromous鱼类如香鱼和galaxids相比，可以反映环境因素如岛尺寸，凉爽的天气或物种的特性。