Due to the crucial role of amino acids in life sciences and pharmaceutics, identification of optical amino acid molecules is of great significance. In this study, the two materials (CNT and PANI) were combined together to obtain the magnification of electrochemical signal by substrate material (CNT/PANI). Then a self‐assembled multiwalled carbon nanotubes/polyaniline/sodium alginate (CNT/PANI/SA) nanocomposite with chiral sites and conductive material was synthesized as the electrochemical sensing interface. Next, a novel electrochemical sensing interface was fabricated via modifying the as‐prepared chiral material on a polished glassy carbon electrode (CNT/PANI/SA/GCE) for precisely, efficiently, and rapidly differentiation of tryptophan (Trp) enantiomers. It was observed that CNT/PANI/SA/GCE showed desirable stereoselective recognition effect in the variety of signal strength to peak current (Ip) to the different optical activity of Trp enantiomers. In the case of optimal conditions, the peak current ratio in the solution of l‐Trp and d‐Trp (I_D/I_L) was observed to be 2.1 at CNT/PANI/SA/GCE by differential pulse voltammogram (DPV). UV–visible spectroscopy further showed that CNT/PANI/SA had a greater binding energy to l‐Trp. Also different factors affecting the enantioselectivity of CNT/PANI/SA/GCE, such as the incubation time, pH, and dropcoating volume of CNT/PANI/SA were optimized. Moreover, the proposed CNT/PANI/SA/GCE showed excellent specific stereoselectivity and anti‐interference ability. Besides, the proposed chiral sensing platform can be effectively applied in real samples to detect Trp enantiomers sensitively. This work inspires us a new path for the preparation of substrate material with excellent electrical conductivity, as well as extend its application potential in chiral recognition.

中文翻译：

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使用自组装多壁碳纳米管/聚苯胺/海藻酸钠复合材料的色氨酸对映体手性伏安传感器

由于氨基酸在生命科学和制药中的关键作用，光学氨基酸分子的鉴定具有重要意义。在本研究中，将两种材料（CNT 和 PANI）结合在一起，通过基板材料（CNT/PANI）获得电化学信号的放大。然后合成了具有手性位点和导电材料的自组装多壁碳纳米管/聚苯胺/海藻酸钠（CNT/PANI/SA）纳米复合材料作为电化学传感界面。接下来，通过在抛光的玻璃碳电极（CNT/PANI/SA/GCE）上修饰制备的手性材料，制造了一种新型的电化学传感界面，以精确、高效、快速地区分色氨酸（Trp）对映异构体。观察到 CNT/PANI/SA/GCE 在各种信号强度到峰值电流 (Ip) 中对 Trp 对映体的不同光学活性显示出理想的立体选择性识别效果。在最优条件下，解的峰值电流比通过差分脉冲伏安图 (DPV)，在 CNT/PANI/SA/GCE 中观察到l -Trp 和d -Trp ( I _D / I _L ) 为 2.1。紫外可见光谱进一步表明 CNT/PANI/SA 对l-Trp。还优化了影响 CNT/PANI/SA/GCE 对映选择性的不同因素，如 CNT/PANI/SA 的孵育时间、pH 和滴涂体积。此外，所提出的 CNT/PANI/SA/GCE 显示出优异的特定立体选择性和抗干扰能力。此外，所提出的手性传感平台可以有效地应用于实际样品中，以灵敏地检测 Trp 对映体。这项工作为我们制备具有优异导电性的基底材料开辟了一条新途径，并扩展了其在手性识别中的应用潜力。