Chromium plays an irreplaceable role in the life system. The exploration of chromium is beneficial to reveal its physiological or pathological processes. In this study, a novel method was established for determination of chromium in gels by matrix-matched external standard method-laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS). The relative standard deviation of chromium signal intensity during ablation standard were less than 16% (n = 300), the correlation coefficient (R²) of LA-ICP-MS quantitative calibration curve was 0.9805, and the detection limit was 2.22 µg/g. The MCF-7 cells were used as an in vitro cell model, and two kinds of polyacrylamide gel electrophoresis (PAGE) method were used to separate the protein extract after chromium incubation. And then, the electrophoresis bands obtained by two electrophoresis methods were ablated respectively by LA-ICP-MS with line scan mode, and the weak chromium-containing protein bands were obtained in electrophoresis band. Finally, the LA-ICP-MS was used to measure and image the chromium in individual cell, and it was found that the cell element distribution map overlapped well with the cell contour map under the laser microscope.

铬在生命系统中起着不可替代的作用。铬的探索有利于揭示其生理或病理过程。在这项研究中，建立了一种通过基质匹配外标法-激光烧蚀电感耦合等离子体质谱法（LA-ICP-MS）测定凝胶中铬的新方法。消融标准时铬信号强度的相对标准偏差小于16％（n = 300），相关系数（R ²LA-ICP-MS定量校准曲线的）为0.9805，检出限为2.22 µg / g。将MCF-7细胞用作体外细胞模型，并使用两种聚丙烯酰胺凝胶电泳（PAGE）方法分离铬孵育后的蛋白质提取物。然后，用线扫描模式分别通过LA-ICP-MS消融通过两种电泳方法获得的电泳带，并在电泳带中获得了较弱的含铬蛋白带。最后，用LA-ICP-MS对单个细胞中的铬进行测量和成像，发现激光显微镜下细胞元素分布图与细胞轮廓图重叠良好。