This study aims to investigate the potential safety hazards and provide reference for improving the medical waste disposal procedure in SARS-CoV-2 testing laboratory. Our SARS-CoV-2 testing group detected the RNA residue on the surface of medical waste with Droplet Digital PCR, and held a meeting to discuss the risks in the laboratory medical waste disposal process. After effective autoclaving, SARS-CoV-2 contaminated on the surface of medical waste bags was killed, but the average concentration of viral RNA residues was still 0.85 copies/cm². It would not pose a health risk, but might contaminate the laboratory and affect the test results. When the sterilized medical waste bags were transferred directly by the operators without hand disinfection, re-contamination would happen, which might cause the virus to leak out of the laboratory. Furthermore, we found that sterilization effect monitoring and cooperation among operators were also very important. In summary, we investigated and analyzed the potential safety hazards during the medical waste disposal process in SARS-CoV-2 testing laboratory, and provided reasonable suggestions to ensure the safety of medical waste disposal.

本研究旨在调查潜在的安全隐患，为改进SARS-CoV-2检测实验室医疗废物处理程序提供参考。我们的SARS-CoV-2检测组通过Droplet Digital PCR检测了医疗废物表面的RNA残留，并召开会议讨论了实验室医疗废物处理过程中的风险。经有效高压灭菌后，医疗垃圾袋表面污染的SARS-CoV-2被杀灭，但病毒RNA残留平均浓度仍为0.85拷贝/cm ². 它不会构成健康风险，但可能会污染实验室并影响测试结果。经消毒的医疗垃圾袋，未经手部消毒，由操作人员直接转运，会造成二次污染，可能导致病毒外泄。此外，我们发现灭菌效果监测和操作人员之间的合作也非常重要。综上所述，我们对SARS-CoV-2检测实验室医疗废物处置过程中存在的安全隐患进行了调查分析，为保障医疗废物处置安全提供了合理建议。