Accurate determination of residual protein content in hydroxypropyl chitin (HPCH), a temperature-sensitive chitin derivative developed recently, is of great importance for biomedical applications. Coomassie brilliant blue (Bradford) method, Lowry method and Bicinchoninic acid (BCA) method were investigated to estimate the content of residual protein in HPCH. It was found that both Bradford and Lowry methods are not suitable for the protein assay in the HPCH sample. The BCA method could be used to determine protein content in HPCH solutions at low concentrations with good accuracy and precision and reasonable limit of detection and limit of quantitation for medical applications.

中文翻译：

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用于生物医学应用的热敏羟丙基甲壳质中蛋白质的定量分析

准确测定最近开发的对温度敏感的几丁质衍生物羟丙基几丁质（HPCH）中的残留蛋白质含量，对生物医学应用非常重要。研究了考马斯亮蓝（Bradford）法，Lowry法和比辛可尼酸（BCA）法，以估算HPCH中残留蛋白质的含量。发现Bradford和Lowry方法均不适用于HPCH样品中的蛋白质测定。BCA方法可用于测定低浓度HPCH溶液中的蛋白质含量，其准确度和精密度高，并且在医疗应用中具有合理的检出限和定量限。