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Nonsterile immunity to cryptosporidiosis in infants is associated with mucosal IgA against the sporozoite and protection from malnutrition
bioRxiv - Microbiology Pub Date : 2021-03-04 , DOI: 10.1101/2021.03.04.433864
Mamun Kabir , Masud Alam , Uma Nayak , Tuhinur Arju , Biplob Hossain , Rubaiya Tarannum , Amena Khatun , Jennifer A. White , Jennie Z. Ma , Rashidul Haque , William A. Petri , Carol A. Gilchrist

We conducted a longitudinal study of cryptosporidiosis from birth to three years of age in an urban slum of Dhaka Bangladesh. Fecal DNA was extracted from monthly surveillance samples and diarrheal stool samples collected from 392 infants from birth to three years. A pan-Cryptosporidium qPCR assay was used to identify sub-clinical and symptomatic cryptosporidiosis. Anthropometric measurements were collected quarterly to assess child nutritional status. 31% (121/392) of children experienced a single and 57% (222/392) multiple infections with Cryptosporidium. Repeat infections had a lower burden of parasites in the stool (Cq slope = -1.85; p<0.0001) and were more likely to be sub-clinical (Chi square test for trend; p=0.01). Repeat infections were associated with the development of growth faltering (Pearson correlation = -0.18; p=0.0004). High levels of fecal IgA antibodies against the Cryptosporidium Cp23 sporozoite protein at one year of life were associated with a delay in reinfection and amelioration of growth faltering through three years of life (HAZ IgA high responders -1.323 ± 0.932 versus HAZ -1.731 ± 0.984 p=0.0001). We concluded that nonsterile immunity to cryptosporidiosis in young children was associated with high levels of mucosal IgA anti-Cp23 and protection from diarrhea and growth faltering.

中文翻译:

婴儿对隐孢子虫病的非无菌免疫力与抗子孢子的粘膜IgA和防止营养不良有关

我们在孟加拉国达卡的贫民窟进行了一项从出生到三岁的隐孢子虫病的纵向研究。从每月监测样本和从出生至三年的392例婴儿中收集的腹泻大便样本中提取粪便DNA。泛隐孢子虫qPCR分析用于鉴定亚临床和有症状的隐孢子虫病。每季度收集人体测量值以评估儿童的营养状况。31%(121/392)的儿童经历了隐孢子虫的单次感染,而57%(222/392)的儿童经历了多次感染。重复感染的粪便中的寄生虫负担较低(Cq斜率= -1.85; p <0.0001),而且更有可能是亚临床的(卡方检验趋势; p = 0.01)。重复感染与生长步履蹒跚有关(Pearson相关系数= -0.18; p = 0.0004)。生命中高水平的针对隐孢子虫Cp23子孢子蛋白的粪便IgA抗体与重新感染和改善生命的步履蹒跚到生命的三年有关(HAZ IgA高应答者-1.323±0.932与HAZ -1.731±0.984 p = 0.0001)。我们得出的结论是,幼儿对隐孢子虫病的非无菌免疫力与高水平的粘膜IgA抗Cp23以及防止腹泻和生长迟缓有关。
更新日期:2021-03-05
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