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Killing of Gram-negative and Gram-positive bacteria by a bifunctional cell wall-targeting T6SS effector
bioRxiv - Microbiology Pub Date : 2021-03-04 , DOI: 10.1101/2021.03.04.433973
Nguyen-Hung Le , Victor Pinedo , Juvenal Lopez , Felipe Cava , Mario Feldman

The type VI secretion system (T6SS) is a powerful tool deployed by Gram-negative bacteria to antagonize neighboring organisms. Here, we report that Acinetobacter baumannii ATCC 17978 (Ab17978) secretes D-lysine (D-Lys), increasing the extracellular pH and enhancing the peptidoglycanase activity of the T6SS effector Tse4. This synergistic effect of D-Lys on Tse4 activity enables Ab17978 to outcompete Gram-negative bacterial competitors, demonstrating that bacteria can modify their microenvironment to increase their fitness during bacterial warfare. Remarkably, this lethal combination also results in T6SS-mediated killing of Gram-positive bacteria. Further characterization revealed that Tse4 is a bifunctional enzyme consisting of both lytic transglycosylase and endopeptidase activities, thus representing a novel family of modularly organized T6SS PG degrading effectors with an unprecedented impact in antagonistic bacterial interactions.

中文翻译:

靶向双功能细胞壁的T6SS效应物杀死革兰氏阴性和革兰氏阳性细菌

VI型分泌系统(T6SS)是革兰氏阴性细菌用来对抗邻近生物的强大工具。在这里,我们报告鲍曼不动杆菌ATCC 17978(Ab17978)分泌D-赖氨酸(D-Lys),增加细胞外pH值并增强T6SS效应子Tse4的肽聚糖酶活性。D-Lys对Tse4活性的这种协同作用使Ab17978能够胜过革兰氏阴性细菌竞争者,表明细菌可以修饰其微环境,从而在细菌战中提高适应性。值得注意的是,这种致命的组合还导致T6SS介导的革兰氏阳性细菌的杀死。进一步的特征表明Tse4是一种双功能酶,由裂解转糖基酶和内肽酶活性组成,
更新日期:2021-03-05
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