Yeasts are considered as potential lipid producer because they are easy to cultivate, able to grow in high cell densities and can produce high concentrations of lipids. Previously we had isolated a promising lipid producing yeast, Zygosaccharomyces siamensis AP1, from wild honey of Central Sulawesi, Indonesia. The strain was able to produce around 19% (w/w biomass) of lipid on glucose. However, its ability to produce lipid on other types of carbon source is still not studied. In this study, we compared the strain ability to produce lipid on glycerol and glucose using different C/N ratio. We also investigated optimum temperature, pH, and growth time for lipid production on glycerol. We found that growing the strain on glycerol with C/N ratio 120 gave the highest lipid content (29.7%). We also found that optimum growth temperature and pH are 30°C and 5, respectively. Maximum lipid biomass, 0.28 g/L, was produced when the strain was grown in the optimum conditions for 72 hours. In conclusion, our results suggest that glycerol is a potential cheap carbon source for lipid production from Z. siamensis AP1. We suggest further study to increase lipid yield either by fermentation or strain improvement.

中文翻译：

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使用甘油作为碳源，从Zygosaccharomyces siamensis AP1产生脂质

酵母被认为是潜在的脂质生产者，因为它们易于培养，能够以高细胞密度生长并且可以产生高浓度的脂质。以前，我们已经分离出了一种很有前途的产脂酵母，即Zygosaccharomyces siamensisAP1，来自印度尼西亚中苏拉威西省的野生蜂蜜。该菌株能够在葡萄糖上产生约19％（w / w生物量）的脂质。然而，其在其他类型的碳源上产生脂质的能力仍未被研究。在这项研究中，我们比较了使用不同C / N比在甘油和葡萄糖上产生脂质的菌株能力。我们还研究了在甘油上产生脂质的最佳温度，pH和生长时间。我们发现在C / N为120的甘油上生长菌株可获得最高的脂质含量（29.7％）。我们还发现最佳的生长温度和pH分别为30°C和5。当菌株在最佳条件下生长72小时后，最大脂质生物量为0.28 g / L。总之，我们的结果表明，甘油是潜在的廉价碳源，可用于从中生产脂质Z.siamensis AP1。我们建议进行进一步的研究以通过发酵或菌株改良来增加脂质产量。