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Spt4 Facilitates the Movement of RNA Polymerase II through the +2 Nucleosomal Barrier
bioRxiv - Genomics Pub Date : 2021-03-04 , DOI: 10.1101/2021.03.03.433772 Ülkü Uzun , Thomas Brown , Harry Fischl , Andrew Angel , Jane Mellor
bioRxiv - Genomics Pub Date : 2021-03-04 , DOI: 10.1101/2021.03.03.433772 Ülkü Uzun , Thomas Brown , Harry Fischl , Andrew Angel , Jane Mellor
Spt4 is a transcription elongation factor, with homologues in organisms with nucleosomes. Structural and in vitro studies implicate Spt4 in transcription through nucleosomes, yet the in vivo function of Spt4 is unclear. Here we assessed the precise position of Spt4 during transcription and the consequences of loss of Spt4 on RNA polymerase II (RNAPII) dynamics and nucleosome positioning in Saccharomyces cerevisiae. In the absence of Spt4, the spacing between gene-body nucleosomes increases and RNAPII accumulates upstream of the nucleosomal dyad, most dramatically at nucleosome +2. Spt4 associates with elongating RNAPII early in transcription and its association dynamically changes depending on nucleosome positions. Together, our data show that Spt4 regulates early elongation dynamics, participates in co-transcriptional nucleosome positioning, and promotes RNAPII movement through the gene-body nucleosomes, especially the +2 nucleosome.
中文翻译:
Spt4促进通过+2核糖体屏障的RNA聚合酶II的运动。
Spt4是一种转录延伸因子,在具有核小体的生物中具有同源性。结构和体外研究表明,Spt4通过核小体转录,但Spt4的体内功能尚不清楚。在这里我们评估了Spt4在转录过程中的精确位置以及Spt4丢失对酿酒酵母中RNA聚合酶II(RNAPII)动力学和核小体定位的后果。在没有Spt4的情况下,基因体核小体之间的间隔增加,RNAPII积累在核小体二元组的上游,最显着的是核小体+2。Spt4在转录早期与延长的RNAPII缔合,其缔合取决于核小体的位置而动态变化。在一起,我们的数据表明Spt4调节早期伸长动态,参与共转录核小体定位,
更新日期:2021-03-05
中文翻译:
Spt4促进通过+2核糖体屏障的RNA聚合酶II的运动。
Spt4是一种转录延伸因子,在具有核小体的生物中具有同源性。结构和体外研究表明,Spt4通过核小体转录,但Spt4的体内功能尚不清楚。在这里我们评估了Spt4在转录过程中的精确位置以及Spt4丢失对酿酒酵母中RNA聚合酶II(RNAPII)动力学和核小体定位的后果。在没有Spt4的情况下,基因体核小体之间的间隔增加,RNAPII积累在核小体二元组的上游,最显着的是核小体+2。Spt4在转录早期与延长的RNAPII缔合,其缔合取决于核小体的位置而动态变化。在一起,我们的数据表明Spt4调节早期伸长动态,参与共转录核小体定位,