Throughout North America, chronic wasting disease (CWD) has emerged as perhaps the greatest threat to wild cervid populations, including white-tailed deer (Odocoileus virginianus). White-tailed deer are the most sought after big game species across North America with populations of various subspecies in nearly all Canadian provinces, the contiguous USA, and Mexico. Documented CWD cases have dramatically increased across the white-tailed deer range since the mid-1990s, including in Minnesota. CWD surveillance in free-ranging white-tailed deer and other cervid populations mainly depends upon immunodetection methods (e.g., immunohistochemistry [IHC] and enzyme-linked immunosorbent assay [ELISA]) on medial retropharyngeal lymph nodes and obex. More recent technologies centered on prion protein amplification methods of detection have shown promise as more sensitive and rapid CWD diagnostic tools. Here, we used blinded samples to test the efficacy of real time quaking-induced conversion (RT-QuIC) in comparison to ELISA and IHC for screening tissues, blood, and feces collected in 2019 from white-tailed deer in southeastern Minnesota, where CWD has been routinely detected since 2016. Our results support previous findings that RT-QuIC is a more sensitive tool for CWD detection than current antibody-based methods. Additionally, a CWD testing protocol that includes multiple lymphoid tissues (medial retropharyngeal lymph node, parotid lymph node, and palatine tonsil) per animal may effectively identify a greater number of CWD detections in a white-tailed deer population than a single sample type (i.e., medial retropharyngeal lymph nodes). These results reveal that the variability of CWD pathogenesis, sampling protocol, and testing platform must be considered for the effective detection and management of CWD throughout North America.

中文翻译：

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慢性浪费性疾病检测方法和程序的比较：对放养的白尾鹿（Odocoileus virginianus）的监视和管理的意义

在整个北美，慢性浪费疾病（CWD）可能已成为野生野生鹿类种群的最大威胁，包括白尾鹿（Odocoileus virginianus）。白尾鹿是北美地区最受欢迎的大型猎物，在几乎所有加拿大省份，邻近的美国和墨西哥都有各种亚种。自1990年代中期以来，包括明尼苏达州，有记录的CWD病例在白尾鹿范围内急剧增加。在自由放养的白尾鹿和其他子宫颈种群中进行CWD监测主要取决于对咽后内侧淋巴结和小便的免疫检测方法（例如，免疫组织化学[IHC]和酶联免疫吸附测定[ELISA]）。以病毒蛋白质扩增检测方法为中心的最新技术已显示出有望成为更灵敏，快速的CWD诊断工具。这里，与ELISA和IHC相比，我们使用盲样本测试了实时地震诱导转化（RT-QuIC）的功效，以筛查2019年从明尼苏达州东南部的白尾鹿采集的组织，血液和粪便，该地区已采用CWD自2016年以来已被常规检测到。我们的结果支持以前的发现，即RT-QuIC比当前基于抗体的方法对CWD检测更敏感。此外，每只动物包括多个淋巴样组织（咽后内侧淋巴结，腮腺淋巴结和p扁桃体）的CWD测试规程可以有效地识别白尾鹿种群中比单个样本类型（即，咽后内侧淋巴结）。这些结果表明，CWD发病机理，采样方案，