Abstract

In this study, to determine whether having potential to be used as hyperaccumulator for Cd and Ni, numerous experiments were designed for conducting assessments for physiological and genotoxic changes along with defining possible alterations on mineral nutrient status of Lemna minor L. by applying Cd-Ni binary treatments (0, 100, 200 and 400 µM). Our study revealed that there were increases in the concentrations of B, Cr, Fe, K, Mg, and Mn whereas decreases were noticed in the concentrations of Na and Zn and the levels of Ca were inversely proportional to Cd-Ni applications showing tendency to increase at the low concentration and to decrease at the high concentration. Randomly Amplified Polymorphic DNA (RAPD) and Inter Simple Sequence Repeat (ISSR) analyses revealed that rather than band losses and new band formations, mostly intensity changes in the band profiles, and low polymorphism and high genomic template stability (GTS) were observed. Although, to date, L. minor was defined as an efficient hyperaccumulator/potential accumulator or competent phytoremedial agent by researchers. Our research revealed that L. minor showing high accumulation capability for Cd and having low polymorphism rate and high genomic template stability is a versatile hyperaccumulator, especially for Cd; therefore, highly recommended by us for decontamination of water polluted with Cd.

NOVELTY STATEMENT

Many studies have been focused on the effects of individual metal ions. However, heavy metal contaminants usually exist as their mixtures in natural aquatic environments. Especially, Cd and Ni coexist in industrial wastes.

In this study, the accumulation properties of Lemna minor for both Cd and Ni were investigated and the effects of Cd and Ni on the bioaccumulation of B, Ca, Cu, Fe, Mg, K, Mn, Na, Pb and Zn in L. minor were also determined. This study furthermore aimed to assess the genotoxic effects of Cd and Ni found in being extended concentrations on DNA using the Randomly Amplified Polymorphic DNA-Polymerase Chain Reaction (RAPD-PCR) method.

摘要

在这项研究中，为了确定是否有可能被用作 Cd 和 Ni 的超富集剂，设计了许多实验来评估生理和遗传毒性变化，以及确定Lemna minor矿物质营养状况的可能变化L. 通过应用 Cd-Ni 二元处理（0、100、200 和 400 µM）。我们的研究表明，B、Cr、Fe、K、Mg 和 Mn 的浓度增加，而 Na 和 Zn 的浓度下降，Ca 的含量与 Cd-Ni 应用成反比，显示出低浓度增加，高浓度减少。随机扩增多态性 DNA (RAPD) 和内部简单序列重复 (ISSR) 分析表明，观察到的不是条带丢失和新条带形成，主要是条带谱中的强度变化，以及低多态性和高基因组模板稳定性 (GTS)。尽管迄今为止，L. minor被研究人员定义为有效的超蓄积剂/潜在蓄积剂或有能力的植物修复剂。我们的研究表明，L. minor对 Cd 具有高积累能力，并且具有低多态性和高基因组模板稳定性，是一种多功能的超富集植物，尤其是对 Cd 的积累；因此，我们强烈推荐用于净化受 Cd 污染的水。

新颖性声明

许多研究都集中在单个金属离子的影响上。然而，重金属污染物通常以其混合物形式存在于天然水生环境中。特别是工业废物中Cd和Ni共存。

在这项研究中，研究了Lemna minor对 Cd 和 Ni的积累特性，以及 Cd 和 Ni 对L中 B、Ca、Cu、Fe、Mg、K、Mn、Na、Pb 和 Zn 的生物积累的影响。未成年人也确定了。本研究还旨在使用随机扩增多态性 DNA-聚合酶链反应 (RAPD-PCR) 方法评估 Cd 和 Ni 在延长浓度时对 DNA 的遗传毒性影响。