An 8-weeks feeding trial was conducted to evaluate the effects of different dietary phosphorus (P) levels on growth performance, P concentrations in tissue, haemolymph biochemistry and hepatopancreatic metabolic enzyme activities for juvenile swimming crab (Portunus trituberculatus). Five semi-purified diets (46 % protein, 7.5 % lipid) were formulated to contain different P levels of 6.4, 10.8, 16.2, 21.0 and 25.7 g kg⁻¹, respectively. Each diet was randomly assigned to triplicate groups of 30 juvenile swimming crab (initial weight was 12.82 ± 0.15 g) that were reared in single crab unit. The results indicated that percent weight gain (PWG) were significantly influenced dietary P levels, the highest PWG were observed in crabs fed the diet containing 16.2 and 21.0 g kg⁻¹ P. Moreover, P contents in muscle, hepatopancreas and carapace significantly increased with dietary P levels increasing from 6.4–21.0 g kg⁻¹. P retention rate (PRR) in hepatopancreas and muscle significantly decreased with dietary P levels increasing from 6.4 to 25.7 g kg^-1. Furthermore, dietary deficient P (such as 6.4 and 10.8 g kg⁻¹ P) levels reduced ATP content, activities of PK and SDH in hepatopancreas. Crabs fed the diet with 6.4 g kg⁻¹ P also showed significantly lower expression of genes involved in electron transport chain complex, such as nd1, sdhc and coxIII in hepatopancreas than those fed the other diets. The expression of genes related to mitochondrial energy metabolisms, such as Atpase6, sirt1, and nrf1 in hepatopancreas were significantly up-regulated in crabs fed 16.2 and 21.0 g kg⁻¹ P diets. Overall, based on the two slope broken-line and quadratic regression analysis of PWG against the dietary total P levels, the optimum dietary P requirement was estimated to be 15.91 and 16.83 g kg⁻¹, respectively.

进行了为期8周的喂养试验，以评估不同日粮磷水平对少年游泳蟹（Portunus trituberculatus）的生长性能，组织中P浓度，血淋巴生化和肝胰腺代谢酶活性的影响。配制了五种半精制日粮（46％的蛋白质，7.5％的脂质）以分别包含6.4、10.8、16.2、21.0和25.7 g kg ^-1的不同P水平。每种饮食被随机分为三组，每组三只，以单个蟹为单位饲养的30只幼年游泳蟹（初始重量为12.82±0.15克）。结果表明，体重增加百分比（PWG）显着影响饮食中的P水平，在饲喂含16.2和21.0 g kg - ¹日粮的螃蟹中观察到最高的PWGP.此外，随着日粮中P含量从6.4-21.0 g kg ^-1增加，肌肉，肝胰腺和甲壳中的P含量显着增加。随着饮食中磷水平从6.4 g kg ^-1增加到25.7 g kg ^-1，肝胰腺和肌肉中的P保留率（PRR）显着降低。此外，饮食中缺乏的P（例如6.4和10.8 g kg ^-1 P）水平降低了肝胰腺中的ATP含量，PK和SDH活性。饲喂6.4 g kg ^-1 P的螃蟹还显示出与电子传输链复合体有关的基因nd1，sdhc和coxIII的表达明显降低。肝胰脏中的食物比其他饮食中的食物多。在喂食16.2和21.0 g kg ^-1 P饲料的螃蟹中，肝胰腺中与线粒体能量代谢相关的基因（如Atpase6，sirt1和nrf1）的表达显着上调。总体而言，基于PWG对饮食中总磷水平的两个斜率折线和二次回归分析，最佳饮食中磷的需求量分别估计为15.91和16.83 g kg ^-1。