The heat shock protein (Hsp70) level was assessed after 14 days of oral gavage-exposure to fumonisin B₁ (FB₁: 150 µg/animal/day), deoxynivalenol (DON: 30 µg/animal/day) and zearalenone (ZEN: 150 µg/animal/day), alone or in combinations (in additive manner: FD = FB₁ + DON, FZ = FB₁ + ZEN, DZ = DON + ZEN and FDZ = FB₁ + DON + ZEN) in the liver, kidneys and lung of 24 adult male Wistar rats (n = 3/group). The liver was the most responsive tissue, as compared with kidney and lung. Except of DZ-treatment, mycotoxins elevated the Hsp70 levels in livers. The highest Hsp70-levels (≈ twofold) were in the DON, FD, FZ and FDZ treatments (additive effects). In the kidney, alterations (↑ ≈ twofold) were detected in ZEN, FD, FZ and DZ treatments. The least responsive organ was the lung (↑ only in FDZ, antagonistic effect). DON and ZEA exposures have altered the reduced glutathione concentration (↓) and glutathione peroxidase activity (↓) in the blood serum. The serum malondialdehyde level increased only after exposure to FD (synergistic effect), as compared with the DZ group (antagonistic effect). When the blood clinical chemistry was assessed, significant alterations were in alanine aminotransferase (80% increase in FDZ, antagonistic effect) and total protein (↓ ZEN). Results varied according to the organ, toxin type and interactions. Furthermore, oxidative stress was not the only key player behind the Hsp70 increase, in which another mechanism is suggested.

经口管饲暴露于伏马菌素 B ₁（FB ₁：150 µg/动物/天）、脱氧雪腐镰刀菌烯醇（DON：30 µg/动物/天）和玉米赤霉烯酮（ZEN： 150 µg/动物/天），单独或组合（以相加方式：FD = FB ₁  + DON、FZ = FB ₁  + ZEN、DZ = DON + ZEN 和 FDZ = FB ₁  + DON + ZEN）在肝脏中， 24 只成年雄性 Wistar 大鼠的肾脏和肺 ( n = 3/组）。与肾脏和肺相比，肝脏是反应最快的组织。除了 DZ 治疗外，霉菌毒素会提高肝脏中的 Hsp70 水平。最高的 Hsp70 水平（≈ 两倍）在 DON、FD、FZ 和 FDZ 处理（附加效应）中。在肾脏中，在 ZEN、FD、FZ 和 DZ 治疗中检测到改变（↑ ≈ 两倍）。反应最弱的器官是肺（↑ 仅在 FDZ，拮抗作用）。DON 和 ZEA 暴露改变了血清中降低的谷胱甘肽浓度 (↓) 和谷胱甘肽过氧化物酶活性 (↓)。与DZ组（拮抗作用）相比，血清丙二醛水平仅在暴露于FD后（协同作用）增加。当评估血液临床化学时，丙氨酸转氨酶发生了显着变化（FDZ 增加了 80%，拮抗作用）和总蛋白（↓ZEN）。结果因器官、毒素类型和相互作用而异。此外，氧化应激并不是 Hsp70 增加背后的唯一关键因素，其中提出了另一种机制。