Chrysanthemum indicum var. aromaticum is an important resource plant for breeding excellent aromatic varieties of ornamental and medicinal chrysanthemums. However, the biosynthesis and regulation mechanisms of typically aromatic ingredients in C. indicum var. aromaticum are poorly understood, which restricted the utilization of this plant. Here, the transcriptome of C. indicum var. aromaticum was investigated and comparative analysis was conducted with the other two closely related chrysanthemum taxa C. indicum and C. nankingense. The global transcriptome profiles showed clear difference of gene expression patterns between the three chrysanthemums, of which 52 flavonoid biosynthesis-associated genes, 157 transcription factors, and 24 flavonoid Uridine diphosphate (UDP) -glucuronosyl and -glucosyltransferase genes were significantly differentially expressed. Furthermore, 22 differential expression genes (DEGs) in relation to the terpenoid backbone biosynthesis and 183 DEGs related to terpenoid diversification were identified. Of which, 14 genes involved into TS/CYP gene cluster showed higher expression in CIA than those in CI or CN. These results increased our understanding of flavonoid diversification and aromatic ingredients accumulation in plants, and will also help improve resource conservation and sustainable utilization of the important resource plant C. indicum var. aromaticum.

菊花标记变种。芳香族是培育观赏和药用菊花优良芳香品种的重要资源植物。然而，典型的芳香成分C.indicum var。的生物合成和调节机制。芳香族了解甚少，这限制了该植物的利用。在这里，C。indicum var的转录组。对芳香族进行了调查，并与其他两种密切相关的菊花紫杉（C. indicum）和楠杉（C. nankingense）进行了比较分析。。全局转录组谱显示三个菊花之间的基因表达模式明显不同，其中52个类黄酮生物合成相关基因，157个转录因子和24个类黄酮尿苷二磷酸（UDP）-葡萄糖醛酸和-葡糖基转移酶基因被显着差异表达。此外，鉴定出与萜类骨架生物合成有关的22个差异表达基因（DEG）和与萜类化合物多样化有关的183个DEG。其中，涉及TS / CYP基因簇的14个基因在CIA中的表达高于在CI或CN中的表达。这些结果加深了我们对植物中类黄酮多样化和芳香成分积累的了解，也将有助于改善重要资源植物的资源节约和可持续利用。C.indicum var。香气。