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Interstitial fluid velocity is decreased around cortical bone vascular pores and depends on osteocyte position in a rat model of disuse osteoporosis
Biomechanics and Modeling in Mechanobiology ( IF 3.5 ) Pub Date : 2021-03-05 , DOI: 10.1007/s10237-021-01438-4
Vittorio Gatti 1 , Michelle J Gelbs 1 , Rodrigo B Guerra 1 , Michael B Gerber 1 , Susannah P Fritton 1
Affiliation  

Muscle paralysis induced with botulinum toxin (Botox) injection increases vascular porosity and reduces osteocyte lacunar density in the tibial cortical bone of skeletally mature rats. These morphological changes potentially affect interstitial fluid flow in the lacunar–canalicular porosity, which is thought to play a role in osteocyte mechanotransduction. The aim of this study was to investigate the effects of disuse-induced morphological changes on interstitial fluid velocity around osteocytes in the bone cortex. Micro-CT images from a previous study that quantified the effects of Botox-induced muscle paralysis on bone microarchitecture in skeletally mature rats were used to create high-resolution, animal-specific finite element models that included the vascular pores and osteocyte lacunae within the tibial metaphysis of Botox-injected (BTX, n = 8) and saline-injected control (CTRL, n = 8) groups. To quantify fluid flow, lacunar and canalicular porosities were modeled as fluid-saturated poroelastic materials, and boundary conditions were applied to simulate physiological loading. This modeling approach allowed a detailed quantification of the fluid flow velocities around osteocytes in a relatively large volume of bone tissue. The analysis demonstrated that interstitial fluid velocity at the vascular pore surfaces was significantly lower in BTX compared to CTRL because of the decreased vascular canal separation. No significant differences in average fluid velocity were observed at the osteocyte lacunae and no correlation was found between the fluid velocity and the lacunar density, which was significantly lower in BTX. Instead, the lacunar fluid velocity was dependent on the osteocyte’s specific position in the bone cortex and its proximity to a vascular pore.



中文翻译:

在废用性骨质疏松症大鼠模型中,皮质骨血管孔周围的间质液速度降低,并取决于骨细胞的位置

肉毒杆菌毒素 (Botox) 注射诱导的肌肉麻痹会增加骨骼成熟大鼠胫骨皮质中的血管孔隙率并降低骨细胞腔隙密度。这些形态变化可能会影响腔隙 - 小管孔隙中的间质液流动,这被认为在骨细胞机械转导中起作用。本研究的目的是研究废用诱导的形态学变化对骨皮质中骨细胞周围间质液速度的影响。先前研究的显微 CT 图像量化了肉毒杆菌毒素诱导的肌肉麻痹对骨骼成熟大鼠骨微结构的影响,用于创建高分辨率的动物特异性有限元模型,其中包括胫骨内的血管孔和骨细胞陷窝注射肉毒杆菌素(BTX,n  = 8) 和盐水注射对照 (CTRL, n = 8) 组。为了量化流体流动,腔隙和小管孔隙度被建模为流体饱和的多孔弹性材料,并应用边界条件来模拟生理负荷。这种建模方法允许对相对大量骨组织中骨细胞周围的流体流速进行详细量化。分析表明,与 CTRL 相比,BTX 中血管孔表面的间质流体速度显着降低,因为血管管分离减少。在骨细胞腔隙中没有观察到平均流体速度的显着差异,并且流体速度和腔隙密度之间没有相关性,BTX 中的腔隙密度显着较低。反而,

更新日期:2021-03-05
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