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A single-molecule electrical approach for amino acid detection and chirality recognition
Science Advances ( IF 13.6 ) Pub Date : 2021-03-03 , DOI: 10.1126/sciadv.abe4365
Zihao Liu 1 , Xingxing Li 2 , Hiroshi Masai 3 , Xinyi Huang 1 , Susumu Tsuda 4 , Jun Terao 3 , Jinlong Yang 2 , Xuefeng Guo 1, 5
Affiliation  

One of the ultimate goals of analytic chemistry is to efficiently discriminate between amino acids. Here we demonstrate this ability using a single-molecule electrical methodology based on molecular nanocircuits formed from stable graphene-molecule-graphene single-molecule junctions. These molecular junctions are fabricated by covalently bonding a molecular machine featuring a permethylated-β-cyclodextrin between a pair of graphene point contacts. Using pH to vary the type and charge of the amino acids, we find distinct multimodal current fluctuations originating from the different host-guest interactions, consistent with theoretical calculations. These conductance data produce characteristic dwell times and shuttling rates for each amino acid, and allow accurate, statistical real-time, in situ measurements. Testing four amino acids and their enantiomers shows the ability to distinguish between them within a few microseconds, thus paving a facile and precise way to amino acid identification and even single-molecule protein sequencing.



中文翻译:

一种用于氨基酸检测和手性识别的单分子电学方法

分析化学的最终目标之一是有效区分氨基酸。在这里,我们使用基于由稳定的石墨烯-分子-石墨烯单分子结形成的分子纳米电路的单分子电学方法证明了这种能力。这些分子结是通过在一对石墨烯点接触之间共价结合具有全甲基化-β-环糊精的分子机器制造的。使用 pH 值来改变氨基酸的类型和电荷,我们发现不同的主客体相互作用产生不同的多峰电流波动,这与理论计算一致。这些电导数据产生每种氨基酸的特征停留时间和穿梭速率,并允许准确、统计实时、原位测量。

更新日期:2021-03-04
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