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Characterization of Brazilian monofloral and polyfloral honey by UHPLC-MS and classic physical-chemical analyses
Journal of Apicultural Research ( IF 1.9 ) Pub Date : 2021-03-04 , DOI: 10.1080/00218839.2021.1886747
Mariana Degaki Archilia 1 , Amandio Augusto Lagareiro Neto 2 , Maria Cristina Marcucci 3 , Roberta Caroline Bruschi Alonso 4 , Thaiana Cristina de Camargo 2 , Ricardo Costa Camargo 5 , Alexandra Christine Helena Frankland Sawaya 1
Affiliation  

Fifty samples of honey, reported by the suppliers as being: orange (Citrus sinensis), eucalyptus (Eucalyptus spp.), coffee (Coffea Arabica), cipo uva (Cissus rhombifolia), quince (Cydonia oblonga), monjoleiro (Acacia polyphylla), mangrove and honeydew were collected from different states of Brazil, between 2014 and 2016, with the aim of studying their physical-chemical properties and chemical composition, searching for markers to determine their floral origin, authenticity, and quality. There is little information on some of these types of honey, such as quince honey, whose chemical characteristics were defined in the present study. Thus, the total phenolic and flavonoid contents, proteins, color, moisture, and electrical conductivity were evaluated. All the samples presented satisfactory results for color and percentage of sugars in relation to the Brazilian legislation, as well as protein concentration; most were within the limit of conductivity established by the Council of the European Union. Only six samples indicated a probability of overheating, having high HMF values. Phenolic compounds were extracted, analyzed by ultra-high-performance liquid chromatography with mass spectrometry and the data extracted and treated by chemometrics. Honey samples classified as quince by the producers were predominantly monofloral and had a distinct chemical marker; abscisic acid. Some of the samples declared as orange and coffee were clearly grouped, however, some samples declared as eucalyptus, coffee, and orange honey were probably not monofloral. Eucalyptus honey had high total phenolics and flavonoids, thus probably has a higher antioxidant function when compared to the other floral origins analyzed herein.



中文翻译:

通过 UHPLC-MS 和经典物理化学分析对巴西单花和多花蜂蜜进行表征

供应商报告的 50 份蜂蜜样品包括:橙子 ( Citrus sinensis )、桉树 ( Eucalyptus spp. )、咖啡 ( Coffea Arabica )、cipo uva ( Cissus rhombifolia )、榅桲 ( Cydonia oblonga )、monjoleiro ( Acacia polyphylla)), 2014 年至 2016 年间从巴西不同州采集了红树林和蜜露,目的是研究它们的理化特性和化学成分,寻找标记以确定它们的花源、真实性和质量。关于其中一些类型的蜂蜜的信息很少,例如榅桲蜂蜜,其化学特性在本研究中得到了定义。因此,评估了总酚类和类黄酮含量、蛋白质、颜色、水分和电导率。根据巴西立法,所有样品的颜色和糖分百分比以及蛋白质浓度均呈现出令人满意的结果;大多数都在欧盟理事会规定的电导率范围内。只有六个样本表明有过热的可能性,具有高 HMF 值。提取酚类化合物,通过超高效液相色谱和质谱分析,提取的数据通过化学计量学处理。被生产商归类为榅桲的蜂蜜样品主要是单花的,并具有明显的化学标记;脱落酸。一些声明为橙子和咖啡的样品被清楚地分组,然而,一些声明为桉树、咖啡和橙子蜂蜜的样品可能不是单花的。桉树蜂蜜的总酚类物质和类黄酮含量高,因此与本文分析的其他花源相比,桉树蜂蜜可能具有更高的抗氧化功能。被生产商归类为榅桲的蜂蜜样品主要是单花的,并具有明显的化学标记;脱落酸。一些声明为橙子和咖啡的样品被清楚地分组,然而,一些声明为桉树、咖啡和橙子蜂蜜的样品可能不是单花的。桉树蜂蜜的总酚类物质和类黄酮含量高,因此与本文分析的其他花源相比,桉树蜂蜜可能具有更高的抗氧化功能。被生产商归类为榅桲的蜂蜜样品主要是单花的,并具有明显的化学标记;脱落酸。一些声明为橙子和咖啡的样品被清楚地分组,然而,一些声明为桉树、咖啡和橙子蜂蜜的样品可能不是单花的。桉树蜂蜜的总酚类物质和类黄酮含量高,因此与本文分析的其他花源相比,桉树蜂蜜可能具有更高的抗氧化功能。

更新日期:2021-03-04
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