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Immunological activities of the aqueous extracts of Cistanche deserticola as a polysaccharide adjuvant for inactivated foot-and-mouth disease vaccines
Food and Agricultural Immunology ( IF 3 ) Pub Date : 2021-03-03 , DOI: 10.1080/09540105.2021.1880551
Ailian Zhang 1 , Xueli Ba 1 , Xiang Weng 1 , Bin Zhao 1 , Danyang Wang 1 , Hui Cao 2 , Jiong Huang 2
Affiliation  

ABSTRACT

Inactivated foot-and-mouth disease (FMD) vaccines generally require adjuvants to enhance the immunogenicity. To test whether the aqueous extracts of Cistanche deserticola (AECD) could be developed into a polysaccharide adjuvant for inactivated FMD vaccines in mice. Experimental vaccines were prepared with inactivated FMD viral antigen plus AECD or inactivated FMD vaccine plus AECD to subcutaneously immunize mice. AECD developed an FMDV-specific humoral response by increasing IgG antibodies and IL-4 in CD4+ T cells and enhanced lymphocyte proliferation and a balanced Th1/Th2 response. Importantly, AECD induced Th1 type immunity by augmenting the activation of T subsets (CD4+, CD8+, CD44+) and the induction of IFN-γ in T lymphocytes, especially CTL response and neutralizing antibodies in sera. In addition, AECD maintained the higher levels of CD80, CD40, MHC-II, CD86 on CD11c+ cells and decreased the Treg frequency. Our results suggested that AECD had the potential utility as an polysaccharide adjuvant for FMD vaccines.



中文翻译:

肉stan蓉肉aqueous的水提取物作为多糖佐剂用于口蹄疫灭活疫苗的免疫活性

摘要

口蹄疫(FMD)灭活疫苗通常需要佐剂来增强免疫原性。为了测试是否的水提取物肉苁蓉(AECD)可能被开发成一种多糖佐剂小鼠灭活口蹄疫疫苗。用灭活的FMD病毒抗原加AECD或灭活的FMD疫苗加AECD制备实验疫苗以皮下免疫小鼠。AECD通过增加CD4 + T细胞中的IgG抗体和IL-4以及增强的淋巴细胞增殖和平衡的Th1 / Th2反应,发展出FMDV特异性的体液反应。重要的是,AECD通过增强T亚群(CD4 +,CD8 +,CD44 +)和T淋巴细胞中IFN-γ的诱导,尤其是血清中的CTL反应和中和抗体。此外,AECD在CD11c +细胞上维持较高水平的CD80,CD40,MHC-II,CD86,并降低了Treg频率。我们的结果表明,AECD作为FMD疫苗的多糖佐剂具有潜在的实用性。

更新日期:2021-03-04
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