Understanding the responses of the microbial community and greenhouse gas (GHG) emissions to the incorporation of different organic amendments is essential for their proper utilization. In this study, laboratory‐incubated microcosm experiments were conducted to investigate the short‐term effects of pine‐wood biochar and lignite‐based amendment on the microbial communities and GHG emissions from agricultural soil. Soils amended at five different application rates were incubated for 19 d under the conditions of 60% water‐filled pore space and 25 °C. Microbial biomass in the amended soil after incubation was measured by the solid colony counting method, and the soil microbial diversity was assayed using a Biolog EcoPlate. The biochar and lignite‐based amendment had distinct effects on the soil microbial communities and GHG emissions. The microbial community growth and utilization of C sources were improved by the biochar but restrained by the lignite‐based amendment in most cases. The biochar and lignite‐based amendment had a minor impact on methane emissions. Carbon dioxide emissions were promoted by the biochar and inhibited by the lignite‐based amendment during the short‐term incubation period. Nitrous oxide emissions decreased with the application rate of biochar but increased with the rate of lignite‐based amendment. The addition of biochar at a rate of 3–4% and lignite‐based amendment at a rate of <1% has the potential to improve soil quality. Salt leaching is required to avoid accumulation when the biochar and lignite‐based amendments are applied. The findings can provide a reference for the application of biochar and lignite‐based amendment in silt loam soil.

中文翻译：

---

生物炭和褐煤基修正案对微生物群落和农业土壤温室气体排放的影响

了解微生物群落和温室气体（GHG）排放对掺入不同有机修正物的反应对于正确利用它们至关重要。在这项研究中，进行了实验室培养的微观实验，以研究松木生物炭和褐煤基改良剂对农业土壤微生物群落和GHG排放的短期影响。在60％充满水的孔隙空间和25°C的条件下，将以五种不同施用量修正的土壤孵育19天。孵化后的土壤经过改良后，通过固体菌落计数法测量了微生物的生物量，并使用Biolog EcoPlate测定了土壤中的微生物多样性。以生物炭和褐煤为基础的改良剂对土壤微生物群落和温室气体排放具有明显的影响。生物炭改善了微生物群落对碳源的生长和利用，但在大多数情况下受到基于褐煤的修正的限制。基于生物炭和褐煤的修正对甲烷排放量影响很小。在短期孵化期间，生物炭促进了二氧化碳的排放，而褐煤基改良剂抑制了二氧化碳的排放。一氧化二氮的排放量随着生物炭施用量的减少而减少，但随着褐煤基改性剂的增加而增加。以3–4％的比例添加生物炭和以<1％的比例添加褐煤基改良剂有改善土壤质量的潜力。当应用基于生物炭和褐煤的改良剂时，需要进行盐浸以避免积累。研究结果可为粉砂壤土中生物炭和褐煤基改良剂的应用提供参考。