Biodiesel is a renewable alternative fuel and glycerol as a main byproduct of the manufacturing process. Lipids could be produced from crude glycerol by using yeasts. The ability of 107 yeast strains to utilize glycerol was screened and 92 of these were selected. 60 strains were determined as a potential for lipid production by Sudan Black B staining. After secondary screening 25 of them showed specific growth rates (OD 600), high biomass production and lipid content. These strains were identified as Pichia cactophila, P. fermentans, P. anomala, Rhodotorula mucilaginosa, R. dairenensis, Clavispora lusitaniae, Saccharomyces cerevisiae, Wickerhamomyces anomalus, Candida glabrata, C. inconspicua, C. albicans, Yarrowia lipolytica with molecular identifications based on ITS and D1/D2 26S rDNA sequences. The results showed that P. cactophila accumulated lipid up to 64.94%, the highest lipid content. C16:0, C18:0, C18:1 and C18:2 essential fatty acids for biodiesel production were detected by GC–MS in the lipids accumulated by all strains. P. cactophila and C. lusitaniae were reported for the first time as lipid-producing yeasts. The results suggest that selected 25 isolates have the ability to grow on crude glycerol and especially P. cactophila produce lipid that has potential use as a feedstock for second generation biodiesel production.

Graphic Abstract

中文翻译：

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新分离的油质酵母从甘油粗品生产脂质：菌株选择，分子鉴定和脂肪酸分析。

生物柴油是一种可再生的替代燃料，甘油是制造过程中的主要副产品。可以通过使用酵母从粗甘油生产脂质。筛选了107个酵母菌株利用甘油的能力，并选择了其中的92个。通过苏丹黑B染色确定60株可能产生脂质。经过二次筛选后，其中25个细胞显示出特定的生长速率（OD 600），高生物量产量和脂质含量。这些菌株被鉴定为毕赤cactophila，发酵毕赤酵母，P.鲳，红酵母mucilaginosa，R. dairenensis，Clavispora葡萄牙念珠菌，酿酒酵母，Wickerhamomyces anomalus，光滑念珠菌，基于ITS和D1 / D2 26S rDNA序列的分子鉴定的C. inconspicua，白色念珠菌，解脂耶氏酵母（Yarrowia lipolytica）。结果表明，仙人掌假单胞菌积累的脂质高达64.94％，是脂质含量最高的。通过GC-MS在所有菌株积累的脂质中检测到了用于生物柴油生产的C16：0，C18：0，C18：1和C18：2必需脂肪酸。P. cactophila和C.葡萄牙念珠菌报道首次作为脂质生产酵母。结果表明，选定的25个分离株具有在粗甘油上生长的能力，尤其是仙人掌假单胞菌产生的脂质，具有用作第二代生物柴油生产的原料的潜力。

图形摘要