In this study, a maize F2:3 population and a population of RILs were constructed from a cross between Dong156 and Dong237, and SSR markers were then used to map QTL. A phenotypic index of the traits (N = 6) commonly related to seed storability, such as the germination energy, germination percentage, germination index, vigor index, seedling length, and simple vigor index generated under artificial aging treatment. Two consistent regions, cQTL-7 on chromosome 7 and cQTL-10 on chromosome 10, were identified by comparing QTL analysis results from these two populations. The four SSR markers (umc1671, phi328175, umc1648 and phi050) linked to cQTL-7 and cQTL-10 were selected using a Chi-squared test. Marker umc1648 exhibited the highest selection efficiency value of 88.89%. The four SSR markers were used to identify genomic regions related to storability in 141 maize inbred lines from NCRPIS. Finally, seven lines were identified that included the two regions consistently associated with superior seed storability. These results indicated that the four SSR markers screened in this study could be used for selection of maize germplasm with a high degree of seed storability.

在这项研究中，从Dong156和Dong237之间的杂交中构建了玉米F2：3种群和RIL种群，然后使用SSR标记来绘制QTL。性状的表型指数（N = 6），通常与种子的耐贮性有关，例如发芽能，发芽率，发芽指数，活力指数，幼苗长短和人工老化处理后产生的简单活力指数。通过比较这两个种群的QTL分析结果，鉴定出两个一致的区域，即7号染色体上的cQTL- 7和10号染色体上的cQTL- 10。连接到cQTL- 7和cQTL- 4的四个SSR标记（umc1671，phi328175，umc1648和phi050）使用卡方检验选择10个。标记umc1648表现出最高的选择效率值88.89％。这四个SSR标记用于鉴定来自NCRPIS的141个玉米自交系中与耐贮性相关的基因组区域。最后，确定了七个品系，其中包括始终与优异的种子可贮藏性相关的两个区域。这些结果表明，在这项研究中筛选的四个SSR标记可用于选择具有较高种子耐贮性的玉米种质。