The manganese doped zinc selenide nanoparticles (ZnSe:Mn NPs) have been synthesized by thermolysis method using oleic acid and oleylamine as capping agents, and 1-octadecene as solvent. Coating of mesoporous silica is done on ZnSe:Mn (ZnSe:Mn@mSilica) which is further functionalized with amine functional groups by treating with (3-aminopropyl)trimethoxysilane. Further pegylation is done to achieve water dispersibility by conjugating carboxyl groups of poly(ethylene glycol) diacid with the amine groups. These pegylated NPs are subsequently treated with ethylenediamine followed by acrylic acid. Conjugation of tris-(hydroxymethyl-aminomethane) is performed by Michael-type addition reaction to afford ZnSe:Mn@mSilica-PEG-Tris-OH. These tris functionalized NPs have exhibited broad emission ranging from 590-620 nm that is an indicative for their suitability in diagnosis and monitoring progress of cancer treatment. To explore the usefulness of increased surface area because of mesoporosity, doxorubicin is loaded on ZnSe:Mn@mSilica-PEG-Tris-OH NPs through silyl ether linkage and evaluated for cytotoxicity against WEHI-164 mouse fibrosarcoma and RAJI human hematopoietic origin cancer cell lines. A decrease in 12% of cell viability of WEHI-164 cells while 30% decrease in RAJI cell lines (IC₅₀ ≈ 45 nM) are observed. This shows that our formulation has more cytotoxic in RAJI cancer cell lines than that of WEHI-164 cancer cells. These results reveal that the formulation has potential for the application in drug delivery and diagnosis in chemotherapeutics.

中文翻译：

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一锅合成发光锰掺杂的ZnSe纳米粒子及其基于二氧化硅的水分散性制剂，用于靶向递送阿霉素

以油酸和油胺为封端剂，以1-十八碳烯为溶剂，通过热解法合成了锰掺杂硒化锌纳米颗粒（ZnSe：Mn NPs）。介孔二氧化硅的涂层是在ZnSe：Mn（ZnSe：Mn @ mSilica）上完成的，而ZnSe：Mn（ZnSe：Mn @ mSilica）通过用（3-氨基丙基）三甲氧基硅烷处理而进一步被胺官能团官能化。通过使聚（乙二醇）二酸的羧基与胺基缀合，进行进一步的聚乙二醇化以实现水分散性。随后将这些聚乙二醇化的NP先后用乙二胺和丙烯酸处理。通过迈克尔型加成反应进行三-（羟甲基-氨基甲烷）的缀合，得到ZnSe：Mn @ mSilica-PEG-Tris-OH。这些由tris官能化的NP表现出590-620 nm的宽发射范围，这表明它们适合诊断和监测癌症治疗的进展。为了探索由于介孔而增加表面积的有用性，将阿霉素通过甲硅烷基醚键连接到ZnSe：Mn @ mSilica-PEG-Tris-OH NP上，并评估其对WEHI-164小鼠纤维肉瘤和RAJI人造血起源癌细胞系的细胞毒性。WEHI-164细胞的细胞活力降低12％，而RAJI细胞系的细胞活力降低30％（IC Mn @ mSilica-PEG-Tris-OH NPs通过甲硅烷基醚键连接，并评估了其对WEHI-164小鼠纤维肉瘤和RAJI人造血起源癌细胞系的细胞毒性。WEHI-164细胞的细胞活力降低12％，而RAJI细胞系的细胞活力降低30％（IC Mn @ mSilica-PEG-Tris-OH NPs通过甲硅烷基醚键连接，并评估了其对WEHI-164小鼠纤维肉瘤和RAJI人造血起源癌细胞系的细胞毒性。WEHI-164细胞的细胞活力降低12％，而RAJI细胞系的细胞活力降低30％（IC₅₀ ≈45 nM）的观察。这表明我们的制剂在RAJI癌细胞系中比WEHI-164癌细胞具有更大的细胞毒性。这些结果表明该制剂具有在化学治疗中的药物递送和诊断中的应用潜力。