Tea black tussock moth, Dasychira baibarana (Matsumura) (Lepidoptera: Lymantriidae), is a devastating pest species of the tea plant in China. Here, we evaluated the responses of D. baibarana to tea plant volatiles using gas chromatography coupled electroantennographic detection (GC–EAD), eleclectroantennography (EAG), and a Y-tube olfactometer. In total, 11 of 18 analyzed compounds elicited GC–EAD responses from test insects. GC–EAD bio-active compounds were further investigated using EAG and behavioral responses. In the EAG analysis, male moths had significantly greater responses to four compounds [(Z)-3-hexenyl butyrate, (Z)-3-hexen-1-ol, ocimene and benzyl alcohol] than female moths. For females, maximum EAG amplitudes, were recorded in response to linalool, (Z)-3-hexenyl hexanoate and (Z)-jasmone. In EAG and behavioral bio-assays, the responses of both sexes were dose independent. In behavioral bio-assays male moths responding significantly to (Z)-3-hexen-1-ol, ocimene, (Z)-3-hexenyl butyrate, linalool, benzyl alcohol, and (Z)-jasmone at various concentrations. For females, significant behavioral responses were observed to (Z)-3-hexenyl hexanoate, followed by (Z)-jasmone, linalool, ocimene, and benzyl alcohol. However, neither sex was sensitive to 4 of the 11 tested compounds, phenyethyl alcohol, phenylacetonitrile, (E)-nerolidol, and indole. The present results showed that tea plant volatiles influenced the behavior of D. baibarana moths, which will greatly contribute in developing eco-friendly control strategies for D. baibarana, through the application of a blend of compounds that showed significant EAG and behavioral responses or a blend combined with female-produced sex pheromones.

中文翻译：

---

Dasychira baibarana (Lepidoptera: Lymantriidae) 对茶树挥发物的电生理和行为反应

茶黑草螟蛾（学名：Dasychira baibarana (Matsumura)）（鳞翅目：lymantriidae）是我国茶树的一种破坏性害虫。在这里，我们使用气相色谱耦合电子天线检测 (GC-EAD)、电子天线 (EAG) 和 Y 管嗅觉仪评估了 D. baibarana 对茶树挥发物的反应。总共 18 种分析化合物中的 11 种引起了测试昆虫的 GC-EAD 反应。使用 EAG 和行为反应进一步研究了 GC-EAD 生物活性化合物。在 EAG 分析中，雄性飞蛾对四种化合物 [(Z)-3-hexenyl butyrate、(Z)-3-hexen-1-ol、罗勒烯和苯甲醇] 的反应明显高于雌蛾。对于女性，最大 EAG 振幅记录响应芳樟醇、(Z)-3-己烯基己酸酯和 (Z)-茉莉酮。在 EAG 和行为生物测定中，两性的反应与剂量无关。在行为生物测定中，雄性飞蛾对不同浓度的 (Z)-3-hexen-1-ol、罗勒烯、(Z)-3-己烯基丁酸酯、芳樟醇、苯甲醇和 (Z)-茉莉酮有显着反应。对于女性，观察到对 (Z)-3-己烯基己酸酯的显着行为反应，其次是 (Z)-茉莉、芳樟醇、罗勒烯和苯甲醇。然而，两种性别都对 11 种测试化合物中的 4 种不敏感，即苯乙醇、苯乙腈、(E)-橙花醇和吲哚。目前的结果表明，茶树挥发物会影响 D. baibarana 飞蛾的行为，这将极大地有助于制定 D. baibarana 的生态友好型控制策略，