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Epigenetic Integrity of Orthodox Seeds Stored under Conventional and Cryogenic Conditions
Forests ( IF 2.9 ) Pub Date : 2021-03-03 , DOI: 10.3390/f12030288
Beata P. Plitta-Michalak , Mirosława Z. Naskręt-Barciszewska , Jan Barciszewski , Paweł Chmielarz , Marcin Michalak

The level of 5-methylcytosine (m5C) in DNA has been observed to change in plants in response to biotic and abiotic stress factors. Little information has been reported on alterations in DNA methylation in orthodox tree seeds in response to storage conditions. In the current study, epigenetic integrity was analyzed in seeds of Pyrus communis L. in response to conventional and cryogenic storage. The results indicate that conventional storage under optimal conditions resulted in a significant increase in m5C. In contrast, a decrease in m5C level after cryostorage at high water content (WC) was observed, not only in seeds but also in 3-month-old seedlings which were smaller than seedlings obtained from seeds cryostored at optimal WC. This shows that non-optimal cryostorage conditions increase epigenetic instability in seeds and seedlings. Optimal procedures for germplasm conservation are very important for germplasm banking since they have serious implications for the quality of stored collections. Maintaining epigenetic integrity during WC adjustment and optimal storage is a characteristic feature of orthodox seeds. The current results underline the importance of proper protocols and techniques for conventional storage and particularly cryopreservation as a method for conservation of true-to-type germplasm for long periods.

中文翻译:

在常规和低温条件下存储的正统种子的表观遗传完整性

已观察到DNA中5-甲基胞嘧啶(m 5 C)的水平响应于生物和非生物胁迫因素而改变。关于存储条件的响应,关于正统树种子DNA甲基化改变的报道很少。在当前的研究中,响应常规和低温储存,分析了Pyrus communis L.种子中的表观遗传完整性。结果表明最佳条件下,传统的存储导致米显著增加5 ℃。在另一方面,在米的降低5不仅在种子中,而且在3个月大的幼苗中,都观察到在高含水量(WC)下冷冻保存后的C水平,该种子的大小小于从以最佳WC冷冻保存的种子获得的幼苗。这表明非最佳的冷冻条件增加了种子和幼苗的表观遗传不稳定。最佳的种质保护程序对于种质库非常重要,因为它们对存储的收集质量有严重的影响。在WC调节和最佳贮藏过程中保持表观遗传完整性是正统种子的特征。目前的结果强调了适当的规程和技术对于常规存储特别是低温保藏作为长期保存真正型种质的方法的重要性。
更新日期:2021-03-03
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