Abstract

Silk fibroin (SF) has excellent biocompatibility, biodegradability and mechanical properties and has been widely applied in the fields of scaffold materials for tissue engineering, drug delivery materials, etc. Mouse embryonic fibroblasts (MEFs), as one of the most commonly used cells with many remarkable properties for modern biomedicine research fields, can serve as the indicator to characterize the biocompatibility of culture materials according to the cell proliferation. In the research described in this paper we studied the effects of SF films formed by various dissolution methods and various kinds of SF materials on the MEFs cell cultivation. Three groups of different SF films were prepared, namely, mulberry SF films prepared by a LiBr dissolution system, mulberry SF films prepared by a HCOOH/CaCl₂ dissolution system and tussah SF films prepared by a HCOOH/CaCl₂ dissolution system. The prepared SF films were characterized for morphology and structure by scanning electron microscopy (SEM) and Fourier transform infrared spectroscopy (FTIR). Further, laser scanning confocal microscopy (LSCM) and cell counting kit-8 (CCK-8) were utilized to examine the growth of the MEFs on the SF films. The experimental results of the three groups of SF films were compared; they showed that the mulberry SF film, prepared by the LiBr dissolution system, had the best biocompatibility and a positive effect on the growth and proliferation of the MEFs.

摘要

丝素蛋白 (SF) 具有优异的生物相容性、生物降解性和机械性能，已广泛应用于组织工程支架材料、药物递送材料等领域。小鼠胚胎成纤维细胞 (MEF) 作为最常用的细胞之一现代生物医学研究领域的许多显着特性，可以作为根据细胞增殖表征培养材料生物相容性的指标。在本文所述的研究中，我们研究了通过各种溶解方法形成的 SF 膜和各种 SF 材料对 MEFs 细胞培养的影响。制备了三组不同的SF薄膜，即LiBr溶解系统制备的桑树SF薄膜，HCOOH/CaCl ₂制备的桑树SF薄膜。溶解系统和由 HCOOH/CaCl ₂溶解系统制备的 tussah SF 薄膜。通过扫描电子显微镜 (SEM) 和傅里叶变换红外光谱 (FTIR) 对制备的 SF 薄膜的形态和结构进行表征。此外，激光扫描共聚焦显微镜 (LSCM) 和细胞计数套件 8 (CCK-8) 用于检查 MEF 在 SF 薄膜上的生长。比较了三组SF薄膜的实验结果；他们表明，由溴化锂溶解系统制备的桑树 SF 膜具有最佳的生物相容性，并且对 MEF 的生长和增殖具有积极作用。