The study aim was to investigate the potential of salivary biochemical assessments to determine smoking cessation in patients under smoking cessation programs. The study validated smoking behavior and degree of nicotine dependence using Fagerström test for nicotine dependence in smokers and non-smokers. Protein components were examined using Raman spectroscopy. Salivary matrix metalloproteinases-8 (MMP-8) levels were analyzed using Quantikine human total MMP-8 immunoassay-kits. Thiocyanate (SCN⁻) concentrations, peroxidase and glutathione-peroxidase activities were determined using spectrophotometric methods. Human mononuclear cell line was examined for M1 polarization. The data were analyzed using Fisher’s exact test and two-tailed paired t-test at α = 0.05. Amide I peak in smoker group was significantly reduced as compared to non-smokers (p < 0.05). Sulfhydryl bond, dialkyl disulfides SS-stretch and corresponding disulphide bond at 500−545 cm^-1 were significantly increased in smokers (p < 0.05). The CH₂ wag showed higher intensity amongst smoker group representing a recoil of carbon atoms. Salivary MMP-8 levels of participants after smoking cessation was significantly lower than smoking salivary levels (p < 0.05). The SCN⁻ and peroxidase concentrations were significantly higher in smoking group whereas an M1 polarization was seen in non-smokers. Salivary biochemical analysis can aid clinicians to verify smoking cessation in patients under smoking cessation programs.

该研究的目的是调查唾液生化评估在确定戒烟计划患者中的戒烟潜力。该研究使用Fagerström检验对吸烟者和非吸烟者的尼古丁依赖性进行了验证，从而验证了吸烟行为和尼古丁依赖性程度。使用拉曼光谱法检查蛋白质成分。使用Quantikine人类总MMP-8免疫测定试剂盒分析唾液基质金属蛋白酶8（MMP-8）的水平。硫氰酸盐（SCN ^-使用分光光度方法测定浓度，过氧化物酶和谷胱甘肽过氧化物酶活性）。检查人单核细胞系的M1极化。使用Fisher精确检验和两尾配对t来分析数据-在α= 0.05时进行测试。与不吸烟者相比，吸烟者组的酰胺I峰值显着降低（p <0.05）。吸烟者的巯基键，二烷基二硫键SS拉伸和500-545 cm ^-1处相应的二硫键显着增加（p <0.05）。CH ₂摇摆物在吸烟者群体中显示出更高的强度，代表碳原子的后坐力。戒烟后参与者的唾液MMP-8水平明显低于吸烟唾液水平（p <0.05）。的SCN ^-和过氧化物酶的浓度在吸烟组而一个M1极化是在不吸烟者均看到更高显著。唾液生化分析可以帮助临床医生验证戒烟计划中患者的戒烟情况。