Royal jelly (RJ) is a natural product that is fed to bee queens during their entire life, and it contributes to their growth and development, fertility, and long lifespan. Although it is known that these unique effects of RJ, it has not been evaluated fed diet studies in fish until now. The study was carried out to determine the influence of RJ diets with different rates (0.0; 0.1; 0.4; 1.6 and 6.4 %) on expressions of growth (GH-I, IGF-I) and immune system (TGF-β) hormones of zebrafish (Danio rerio). Growth parameters was also studied and found to be higher in D4 group containing 1.6 % additives compared with other groups. RJ dietary supplementation showed significantly up-regulated in GH-1 gene expression in the liver tissues of fish fed D5 diet respect to the control group. Conversely, expression level was down-regulated in kidney tissues of group D4 compared with the control group (p < 0.05). IGF-1 gene expression noticeably increased in the muscle tissues of fish fed the D5 diet containing 6.4 % RJ compared to the control group. The greatest decrease was found in the muscle tissues of group D3 diet (p < 0.05). Although among the diet groups the highest amount of TGF-β expression was found in the muscle tissue of fish that were fed the D4 diet. It was seen that the mRNA level amounts obtained in all applications were below the level of the control group. These results indicate that the high-dose RJ included in the feed positively influenced the growth hormones of the zebrafish at transcriptomic level. To sum up, this study highlights the importance that 1.6 % or 6.4 % RJ included in the diet of zebrafish positively influenced their growth performance and growth hormone gene expression and may be considered a potential to be sustainable alternative for growth promoter in aquaculture practice.

蜂王浆（RJ）是一种天然产品，在蜂王一生中都被喂食，它有助于蜂王的生长发育，繁殖力和长寿命。尽管已知RJ具有这些独特的作用，但到目前为止，还没有在鱼类的饮食研究中对RJ进行过评估。该研究旨在确定不同比率（0.0; 0.1; 0.4; 1.6和6.4％）的RJ饮食对生长激素（GH-I，IGF-I）和免疫系统（TGF- β）激素表达的影响。斑马鱼（斑马鱼）。还研究了生长参数，发现与其他组相比，含1.6％添加剂的D4组的生长参数更高。与对照组相比，RJ膳食补充剂显示饲喂D5饲料的鱼肝组织中GH-1基因表达显着上调。相反，与对照组相比，D4组肾脏组织中的表达水平下调（p <0.05）。与对照组相比，喂食含6.4％RJ的D5日粮的鱼的肌肉组织中IGF-1基因表达显着增加。在D3组饮食的肌肉组织中发现最大的下降（p <0.05）。尽管在饮食组中，以D4饮食喂养的鱼的肌肉组织中TGF-β的表达量最高。可以看出，在所有应用中获得的mRNA水平量均低于对照组水平。这些结果表明，饲料中包含的高剂量RJ在转录组水平上对斑马鱼的生长激素产生了积极影响。总而言之，这项研究强调了斑马鱼饮食中所含的1.6％或6.4％RJ对它们的生长性能和生长激素基因表达产生积极影响的重要性，在水产养殖实践中，它可能被认为是可持续的替代生长促进剂的潜力。