In the present study, the sensitivity of the gut microbes of dengue fever mosquito Aedes aegypti to Indian traditional medicinal plants was evaluated. The microbes were isolated from the midgut of laboratory-reared fourth instar larvae of Ae. aegypti and grown on Luria-Bertini agar plates at an optimum temperature of 27ºC. The microbial colonies were differentiated based on their characteristics such as size, shape, opacity, elevation, consistency, and rate of growth. The axenic culture of different strains was obtained by streaking method. Ten different types of microbial clones were identified up to species level using Biolog’s advanced phenotypic technology. Five Indian traditional plants: Ocimum sanctum, Azadirachta indica, Catharanthus roseus, Curcuma longa, and Syzygium aromaticum and an invasive obnoxious weed Lantana camara were used in the present study. The extracts of the specific parts of individual plants were prepared in ethanol and hexane using ‘Soxhlet apparatus’. The extracts were screened for their antimicrobial activities by disc diffusion assay. The results indicate that the plants used in the present study possessed antimicrobial activities against gut microbes of Ae. aegypti. However, the sensitivity of different microbes to the extracts varied. The zone of inhibition observed after 24 h of incubation revealed that ethanol extract of A. indica has the most potent antibacterial activity; followed by that of S. aromaticum, C. longa and O. sanctum. L. camara and C. roseus were least effective against gut microbes of Ae. aegypti.

在本研究中，评估了登革热蚊埃及伊蚊的肠道微生物对印度传统药用植物的敏感性。从实验室饲养的Ae四龄幼虫的中肠中分离出微生物。aegypti并在Luria-Bertini琼脂平板上于27ºC的最佳温度下生长。根据微生物菌落的特征（例如大小，形状，不透明度，高度，稠度和生长速率）对其进行区分。通过条纹法获得了不同菌株的树胶培养物。使用Biolog的先进表型技术，可以鉴定出多达10种不同类型的微生物克隆，直至物种水平。印度的五种传统植物：圣殿，本研究使用了印度印，（Azadirachta indica），长春花（Catharanthus roseus），姜黄（Curcuma longa）和蒲桃（Syzygium aromaum），以及入侵性杂草马Lan丹（Lantana camara）。使用“ Soxhlet仪器”在乙醇和己烷中制备单个植物特定部位的提取物。通过椎间盘扩散测定法筛选提取物的抗微生物活性。结果表明，本研究中使用的植物具有针对Ae肠道微生物的抗菌活性。伊蚊。但是，不同微生物对提取物的敏感性各不相同。孵育24小时后观察到的抑制区显示印度A草的乙醇提取物具有最强的抗菌活性；其次是芳香沙门氏菌，长角沙棘和圣殿。L. camara和C. roseus对Ae的肠道微生物影响最小。伊蚊。